槲皮素复合益生元固体饮料的研制及其对小鼠肠道菌群的影响研究

Development of quercetin composite prebiotic solid beverage and its effect on intestinal flora of mice

以槲皮素和低聚果糖为主要原料,利用单因素及正交试验方法确定槲皮素复合益生元固体饮料的最佳配方,并动态观察槲皮素复合益生元固体饮料对肠球菌、肠杆菌、双歧杆菌和乳杆菌体外增殖的影响。结果表明,槲皮素复合益生元固体饮料最佳配方为低聚果糖添加量8 g、槲皮素添加量0.4 g、柠檬酸添加量0.06 g、羧甲基纤维素添加量0.15 g,所得固体饮料具有槲皮素清香、酸甜适度、色泽均匀等良好的感官品质。体外菌群增殖试验表明,槲皮素复合益生元固体饮料能抑制肠球菌和肠杆菌的体外增殖,并且能有效促进双歧杆菌和乳杆菌的体外增殖。

Using quercetin and fructo oligosaccharide as the main materials,the optimal formula of quercetin composite prebiotic solid beverage was determined by the single-factor test and orthogonal test.Effects of quercetin composite prebiotic solid beverage on the proliferation of Enterococcus,Enterobacter,Bifidobacterium and Lactobacillus were dynamically inspected.The experiment proved that the best formula of quercetin composite prebiotic solid beverage is 8 g fructo oligosaccharide,0.4 g quercetin,0.06 g citric acid and0.15 g carboxymethyl cellulose.The beverage has good sensory qualities such as quercetin fragrance,moderate sourness and sweetness,and uniform color.In vitro flora proliferation tests show that fructo oligosaccharides,quercetin,and the quercetin composite prebiotic solid beverage can inhibit the proliferation of Enterococcus and Enterobacter,and can effectively promote the proliferation of Bifidobacterium and Lactobacillus.