汉族健康妊娠妇女HLA-G 3′UTR基因多态性及单倍型的分布特征

Distribution characteristics of HLA-G 3′UTR gene polymorphism and haplotype in Han healthy pregnant women

目的分析人类白细胞抗原G 3′非翻译区(HLA-G 3′UTR)基因多态性及单倍型在本地区汉族正常妊娠妇女中的分布特点。方法选取该院2017年10月至2018年10月产前检查的汉族健康妊娠妇女168例,采集外周血标本,提取全血DNA,利用PCR法扩增HLA-G 3′UTR基因,Sanger测序法检测基因序列,并进行多态性位点基因分型。结果共检测到HLA-G 3′UTR基因8个多态性位点(14 bp ins/del、+3003C/T、+3010G/C、+3027C/A、+3035C/T、+3142C/G、+3187A/G、+3196C/G),均符合Hardy-Weinberg平衡检验(P>0.05)。等位基因以14 bp del、+3003T、+3010G、+3027C、+3035C、+3142C、+3187G、+3196C为主,基因型以14 bp del/del、+3003TT、+3010GC、+3027CC、+3035CC、+3142CG、+3187AG、+3196CC为主。连锁不平衡分析显示8个位点紧密连锁,UTR-1(DTG CCC GC)、UTR-3(DTC CCG AC)、UTR-7(ITC ATG AC)频率大于0.03,三者共占94.3%,其中UTR-1占55.0%。结论温州地区健康妊娠妇女HLA-G 3′UTR检测到8个多态性位点,且8个位点紧密连锁,构建的单倍型UTR-1(DTG CCC GC)是该地区妊娠妇女人群HLA-G 3′UTR主要的单倍型。

Objective To investigate the distribution of human leukocyte antigen-G 3′untranslated region(HLA-G 3′UTR)polymorphism and haplotype in Han healthy pregnant women in this area.Methods A total of 168 Han healthy pregnant women were selected from October 2017 to October 2018 in this hospital.The peripheral blood samples were collected and the whole blood DNA was extracted.The HLA-G 3′UTR gene was amplified by PCR.The gene sequence was detected by Sanger sequencing,and the polymorphic loci were genotyped.Results 8 polymorphic loci of HLA-G 3′UTR gene(14bp ins/del,+3003C/T,+3010G/C,+3027C/A,+3035C/T,+3142C/G,+3187A/G and+3196C/G)were detected.They were all in accord with Hardy-Weinberg equilibrium(P>0.05).14bp del,+3003T,+3010G,+3027C,+3035C,+3142C,+3187G,+3196C were the main alleles,and 14bp del/del,+3003TT,+3010GC,+3027CC,+3035CC,+3142CG,+3187AG,+3196CC were the main genotypes.Linkage disequilibrium analysis showed that 8 loci were closely linked.The frequencies of UTR-1(DTG CCC GC),UTR-3(DTC CCG AC),UTR-7(ITC ATG AC)were greater than 0.03,accounting for 94.3%of the total,in which UTR-1 accounted for 55.0%.Conclusion Eight polymorphic loci of HLA-G 3′UTR gene were found in Han healthy pregnant women in this area,which were closely linked.The constructed haplotype UTR-1(DTG CCC GC)was the main haplotype of HLA-G 3′UTR in pregnant women in this area.