野野村放线菌ATCC 39727利用短链羧酸合成细胞脂肪酸

Evaluation of cell fatty acid biosynthesis in Nonomuraea sp.ATCC 39727 using short-chain carboxylic acids

本实验对野野村放线菌ATCC 39727利用外源添加的不同短链羧酸为起始物合成细胞脂肪酸的能力进行了研究,结果表明,该菌优先利用异丁酰CoA、丁酰CoA和丙酰CoA为细胞脂肪酸合成起始物,但利用3-甲基丁酰CoA为脂肪酸合成起始物的能力较差。细胞脂肪酸中检出了以2-甲基丁酰CoA为起始物合成的anteiso-C17:0脂肪酸,但外源添加2-甲基丁酸却导致anteiso-C17:0脂肪酸质量分数降低。根据研究结果推测,野野村放线菌ATCC 39727细胞脂肪酸的合成取决于合成起始物的量及对脂肪酸合成酶的亲和性。

Biosynthesis of cell fatty acid in Nonomuraea sp.ATCC 39727 using different short-chain carboxylic acids as chain initiators was examined.The results indicated that Nonomuraea sp.ATCC 39727 preferred isobutyryl-CoA,butyryl-CoA and propionyl-CoA as chain initiators for fatty acid biosynthesis,and 3-methylbutyryl-CoA was a poor substrate for the fatty acid synthase.Anteiso-C17:0 acid primed by 2-methylbutyrate was detected in Nonomuraea sp.,however,addition of 2-methylbutyric acid resulted in a slight decrease of anteiso-C17:0 acid.Cell fatty acid biosynthesis in Nonomuraea sp.ATCC 39727 depended on the combination of chain initiator availability with the affinity of the initiators for the fatty acid synthase.