摘要
The present study evaluated the nutritional and total phenolic contents of leaves and fruit of Breynia retusa to study the inhibitory potentials against free radicals,-amylase and-glucosidase enzymes.Proximate composition and anti-nutrient content were investigated in the powdered samples of leaf and fruit.Total phenolic,flavonoid,vitamin C and E contents were measured by spectrophotometric methods.Antioxidant activity was estimated by employing various in vitro assays.The-amylase and-glucosidase inhibitors were studied to determine the anti-diabetic potential of B.retusa.Nutritional composition of fruit showed higher starch,total proteins,total free amino acids and mineral contents as compared to leaves.Anti-nutrient contents of fruit and leaves exhibited a minimum amount of trypsin inhibiting activity and tannin contents.Further,higher contents of total phenolics and vitamin E were found in leaf methanol extracts.The leaf extract also showed higher antioxidant activity in DPPH•,FRAP,metal ion chelating and lipid peroxidation inhibition assays.B.retusa leaf and fruit methanol extracts inhibited-amylase and-glucosidase enzymes.In conclusion,nutritional composition,antioxidant and anti-diabetic activities of B.retusa are suitable and promising for the development as a safe food product or natural additive.
