摘要
The underground tuber of Dioscorea alata is a palatable food and is also claimed to have therapeutic effects.Various groups of researchers demonstrated different activities of this tuber such as antioxidant,hepatoprotective,anti-ulcer and anti-diabetic activities.Therefore,the aim of the present study was to evaluate the immunomodulatory potentialities of 70%hydro-methanolic extract of D.alata underground tubers by investigating different parameters like the expression of interferon gamma(IFN-),interleukin(IL)-2,IL-4 and IL-10,in addition to its effect on the proliferation of murine splenic T-lymphocytes in vitro.Results demonstrated that D.alata can actively polarize the TH0 lymphocyte population towards the expression of TH1 immune response by up-regulating the IFN-and IL-2 expression and down-regulating the IL-4 and IL-10 expression.The tuber extract also proved to possess mitogenic activity as evidenced by the proliferation of lymphocytes in vitro.©2014 Beijing Academy of Food Sciences.Production and hosting by Elsevier B.V.All rights reserved.
基金
The financial assistance was provided by Department of Biotechnology,Government of India(sanction order no.BT/09/NE/TBP/2010).The authors are thankful to Prof.Anupam Chatterjee of the Department of Biotechnology&Bioinformatics,North-Eastern Hill University for supplying the plant material and to Dr.Nripendranath Mandal,Division of Molecular Medicine,Bose Institute for his kind help in preparation of the plant extract.
