鹿血清预处理对大鼠心肌细胞缺糖损伤的保护作用

Protective activities against induced cardiomyocyte injury by deer serum

目的:研究鹿血清预处理对心肌细胞缺糖损伤的影响及作用机制。方法:心肌细胞采用无糖DMEM培养基培养18 h造成缺糖损伤,模拟心肌缺血再灌注实验,观察鹿血清预处理6和12 h两种情况是否减轻缺糖损伤的心肌细胞,并选用羊血清作对照。按随机分组法分为正常对照组、模型组、鹿血清组(5、10和20 mg/mL)及羊血清组(5、10和20 mg/mL),观察各组细胞的形态学变化,检测心肌细胞相对活力,细胞培养液中乳酸脱氢酶(LDH)、丙二醛(MDA)浓度以及谷胱甘肽过氧化物酶(GSH-PX)和超氧化物歧化酶(SOD)活力等指标。结果:预处理6 h情况下,与模型组、正常对照组相比,不同浓度鹿血清及羊血清均显著增加心肌细胞相对活力(P<0.01),鹿血清与羊血清组间的差异无统计学意义(P>0.05)。预处理12 h情况下,不同浓度(5、10、20 mg/mL)鹿血清组心肌细胞相对活力高于模型组(P<0.01),但低于正常对照组(P<0.05);不同浓度(5、10、20 mg/mL)羊血清组心肌细胞相对活力与模型组间的差异无统计学意义(P>0.05);20 mg/mL鹿血清组心肌细胞相对活力高于20 mg/mL羊血清组(P<0.01);与模型组比较,不同浓度(5、10、20 mg/mL)鹿血清组细胞培养液中LDH浓度降低(P<0.01),20 mg/mL鹿血清组及不同浓度(5、10、20 mg/mL)羊血清组GSH-PX活性升高(P<0.01),10 mg/mL鹿血清组SOD活性升高(P<0.01)。结论:鹿血清可保护心肌细胞缺糖损伤,其机制可能与对抗氧自由基损伤及稳定细胞膜有关。

OBJECTIVE:To investigate protective potential of deer serum on cardiomyocyte injury which was induced by glucose deprivation.METHODS:Cardiomyocytes were cultured in vitro and were subjected to glucose deprivation for 18 h to induce myocardial cell injury.An objective was to observe whether deer serum would alleviate the injury by 6 h and 12 h preconditioning,using sheep serum as comparison.The cardiomyocyte cultures were divided into several groups:normal control,model,deer serum-treated(5、10 and 20 mg/mL)and sheep serum-treated(5,10 and 20 mg/mL)groups.Effects of cell morphology,relative activity of myocardial cells,lactate dehydrogenase(LDH)leakage,malondialdehyde(MDA)content,glutathione peroxidase(GSH-Px)and superoxide dismutase(SOD)activity were used as evaluation indexes.RESULTS:Compared with the model and the normal control groups,the 6 h pretreatment by both sera significantly increased the relative activities of cardiomyocytes at different concentrations(P<0.01),but there was no significant difference between the deer and the sheep serum groups(P>0.05).After pretreatment for 12 h with different concentrations of deer serum,the relative activities of myocardial cells were significantly increased over the model group(P<0.01),but the increases were lower than that of the normal control group(P<0.05).There was no statistical significance in activities after treatment with the different concentrations of sheep serum(P>0.05).The relative activities of myocardial cells in the 20 mg/mL deer serum group was significantly higher than that in the 20 mg/mL sheep serum group(P<0.01).On the other hand,the concentrations of LDH in the deer serum groups was significantly decreased compared with the model group(P<0.01).Activities of GSH-Px in the 20 mg/mL deer serum group and in different concentrations(5,10,20 mg/mL)of the sheep serum groups were significantly increased(P<0.01),and activities of SOD in the 10 mg/mL deer serum group was significantly increased(P<0.01).CONCLUSION:Deer serum preconditioning had a protective effect against cardiomyocyte apoptosis which was induced by glucose deprivation.The mechanism may be related to protection against oxygen free radical damage and stabilization of cell membrane.