年龄和性别对正常人外周血淋巴细胞核质桥自发率的影响

Effects of age and gender on formation of nucleoplasmic bridges in peripheral blood lymphocytes

目的:探索年龄、性别因素对我国健康人群外周血淋巴细胞核质桥自发率的影响,为核质桥的影响因素研究提供科学依据。方法:选取98例健康成人,其中男性52例,女性46例,年龄范围为20~68岁,平均年龄为(40.7±12.6)岁。按年龄分为20~29岁组(22人)、30~39岁组(23人)、40~49岁组(25人)和50岁以上组(28人)。抽取研究对象外周血2 mL,采用胞质分裂阻滞法培养细胞40 h后,加入松胞素B(终浓度为10μg/mL),继续培养至68 h收获细胞。每个受试者观察1000个双核细胞,分析核质桥、微核及核芽的数量。结果:核质桥总体自发率为每个细胞0.56‰,男性略高于女性,但差异无统计学意义(P>0.05)。男性在不同年龄组间,核质桥自发率差异无统计学意义(P>0.05)。女性40~49岁年龄组核质桥自发率最高,显著高于20~29岁组(U=2.31,P<0.05)。微核自发率女性高于男性(U=4.40,P<0.05),男性和女性受试者40~49岁组微核自发率均为最高。核芽自发率在不同性别间的差异无统计学意义(P>0.05),在不同年龄组间无明显变化规律。结论:健康人群中的核质桥自发率较低,不受性别因素影响,在20~49岁范围内具有随年龄增加而升高的趋势。

OBJECTIVE:To investigate influence of age and gender on spontaneous formation of nucleoplasmic bridges(NPB)in peripheral blood lymphocyte from in healthy Chinese subjects.METHODS:A total of 98 healthy adults(52 male and 46 female)aged 20-68 years(40.7±12.6 years)were enrolled.The subjects were divided into different age groups:20-29(22 subjects),30-39(23 subjects),40-49(25 subjects)and over 50(28 subjects).At 40 h of their lymphocyte cultures in the cytokinesis-block cytome assay,cytochalasin-B(10μg/mL)was added and cultures were harvested at 68 h.The presence of NPB,micronucleus(MN)and nuclear bud(NBD)in their peripheral blood lymphocytes were determined by analyzing 1000 binucleated cells in each sample.RESULTS:The overall NPB spontaneous rate was 0.56‰,but the rates were non-significantly higher in males than in females.In addition,there was no statistically significant difference in male subjects among different age groups.However,the highest NPB spontaneous rate in females was found in 40-49 age group which was significantly higher than that in the 20-29 age group(U=2.31,P<0.05).The MN spontaneous rates in females were higher than that in males(U=4.40,P<0.05)and the highest rates in both male and female subjects were found in the 40-49 age groups.There was no statistical difference of NBD spontaneous rates between genders and among different age groups.CONCLUSION:NPB spontaneous rate in healthy population was very low which was not affected by gender but had increased trend within the 40-49 age group.