摘要
Organisms can be engineered to produce a wide variety of compounds by either enhancing endogenous metabolic pathways,or by introducing exogenous pathways that are either borrowed from other organism,or de novo-designed pathways unknown to nature.While overexpression of bottleneck enzymes and deletion of competing pathways remain at the core of metabolic pathway engineering,there are many other key elements that need to be considered to successfully develop strains for the production of valuable products.
