番茄过氧化氢酶CRISPR/Cas9敲除突变体的构建

Establishment of catalase CRISPR/Cas9 knockout vector system in tomato

番茄是一种重要的全球经济作物,其产量和品质往往受到病虫害的严重影响,因此获得抗病能力强的番茄新品种一直被认为是番茄稳产和高产的重要途径。光呼吸途径代谢产生的H2O2被广泛地认为是一种重要的抗病信号分子,然而能否提高番茄的抗病能力还不是很清楚。文章采用新型的基因编辑技术CRISPR/Cas9敲除番茄过氧化酶基因,该酶是清除H_2O_2的关键代谢酶,酶活测定显示成功获得7颗番茄过氧化氢酶下调突变体,该遗传材料为后续分析番茄抗病以及果实产量提供了宝贵的遗传资源。

Tomato is an important global economic crop.Its yield and quality are often seriously affected by pests and diseases.Therefore,new tomato varieties with strong disease resistance have been considered as an important way for stable and high yield of tomato.H 2O 2 produced by photorespiration pathway metabolism is widely regarded as an important disease-resistant signaling molecule.However,it is not clear whether it can improve the disease resistance of tomato.In this paper,a new gene editing technology CRISPR/Cas9 was used to knock out the tomato peroxidase gene,which is a key metabolic enzyme for the removal of H 2O 2.The enzyme activity assay showed that seven tomato catalase down-regulated mutants were successfully obtained.These genetic materials provide a valuable genetic resource for subsequent analysis of tomato disease resistance and fruit yield.