摘要
以黑曲霉H-08为出发菌株,通过紫外线(UV)和亚硝基胍(NTG)复合诱变技术,筛选到1株酶活力提高明显的菌株UN3-36。经过多次复筛验证,证明其遗传性质稳定,酶活力显著提高,摇瓶的发酵酶活力可以达到19200 U/m L,较出发菌株提高了90%左右。
The original strain of Aspergillus niger H-08 was modified in genetic characteristics with compound mutagenesis by UV and NTG.A high glucoamylase-yielding strain UN-36was screened from the mutants by slab screening and shake flask rescreening,which has high enzyme activity and good genetic stability.The enzyme activity of the shake flask-fermentationreached 19200 u/ml,which was 50%higher than that of the original strain.
作者
王克芬
宋静静
王金余
王兴吉
刘胜利
刘文龙\
Wang Kefen;Song Jingjing;Wang Jinyu;Wang Xingji;Liu Shengli;Liu Wenglong(Shandong Long Kete Enzyme Co.,ltd.,Shandong Province Key Laboratory of Enzyme Preparation Fermentation Technology,Linyi 276400,China)
出处
《中国饲料添加剂》
2020年第12期12-15,共4页
China Feed Additive
关键词
糖化酶
UV
NTG
复合诱变
筛选
Glucoamylase
UV
NTG
Compound Mutagenesis
Screening
