白藜芦醇调节卵清蛋白诱导的小鼠过敏性鼻炎的作用机制研究

Therapeutic effects of resveratrol on OVA-induced allergic rhinitis in mice and immune mechanisms

目的探讨白藜芦醇对过敏性鼻炎小鼠的行为学、鼻黏膜组织形态学、血清过敏细胞因子和脾脏中Th2型细胞比例的影响。方法小鼠随机分为NC、AR、白藜芦醇低剂量组(30 mg·kg^-1,RL)、白藜芦醇高剂量组(100 mg·kg^-1,RH)、地塞米松组(Dex),共5组。治疗结束后,记录小鼠挠鼻和打喷嚏次数;HE染色观察鼻黏膜炎细胞浸润;ELISA检测血清中IL-4、IL^-10、IL^-13、IL^-17、IFN-γ和OVA-sIgE的水平;流式细胞术检测脾脏中CD4+GATA3+T细胞比例。结果同AR组比较,RH组小鼠挠鼻和打喷嚏次数显著减少,鼻黏膜炎性细胞浸润降低,血清中IL-4、IL^-13和OVA-sIgE水平均显著下降;RH组中小鼠脾脏中CD4+GATA3+Th2比例显著降低。结论高剂量白藜芦醇治疗后,可以降低卵清蛋白诱导的过敏性鼻炎小鼠脾脏中CD4+IL-4+Th2型T细胞比例,降低血清中OVA-sIgE、IL-4、IL^-13水平,缓解小鼠鼻黏膜炎性细胞浸润,进而减少小鼠的挠鼻和打喷嚏次数,缓解小鼠过敏性鼻炎相关症状。

Aim To investigate the curative effects of resveratrol on OVA induced allergic rhinitis in miceand the underlying immune mechanisms.Methods Balb/c mice(female,6 weeks)were divided randomly into normal control(NC)group,allergic rhinitis(AR)group,high dose resveratrol treatment group(RH),low dose resveratrol treatment group(RL),and dexamethasone treatment group(Dex).RL,RH and Dex group were oral administered with resveratrol 30 mg·kg^-1,resveratrol 100 mg·kg^-1 and dexamethasone 10 mg·kg^-1,respectively.After the treatment,the sneezing and nasal rubbing behaviors of mice in all the group were recorded and HE was performed to assess the inflammatory cell infiltration in nasal tissues.The sera levels of allergic cytokines were determined with ELISA assay.The percentage of CD4+GATA3+T cells in spleen of each group was further recorded by flow cytometry.Results Compared with AR group,treatment with resveratrol(100 mg·kg^-1)reduced the sneezing and nasal rubbing behaviors significantly and improved inflammatory cell infiltration in nasal tissues.The up-regulated sera levels of IL-4,IL^-13 and OVA-sIgE in AR group were reversed by RH,and ratios CD4+GATA3+Th2 cells in spleen of RH were also down-regulated parallelly.Conclusions RH treatment could improve the allergic related symptoms of OVA-induced allergic rhinitis,which is associated with down-regulated sera levels of IL-4,IL^-13 and OVA-sIgE and ratios of CD4+GATA3+Th2 cells in spleen of mouse model.