DOX调控HNRNPK稳定下调的H1299细胞株的构建与研究

Establishment and analysis of the H1299 cell line for HNRNPK downregulated by DOX

目的构建DOX调控HNRNPK稳定下调的H1299细胞株,并研究HNRNPK下调对细胞增殖与凋亡的影响。方法用包装好的慢病毒感染H1299细胞,感染72 h后利用嘌呤霉素筛选阳性细胞并扩大培养获得稳转株,通过定量PCR与Western blot检测HNRNPK表达效率,并研究HNRNPK下调对细胞增殖与凋亡的影响。结果成功构建了DOX调控HNRNPK稳定下调的H1299细胞株,HNRNPK的下调抑制了H1299细胞的增殖,但对其凋亡没有明显影响。结论 HNRNPK下调抑制了H1299细胞的增殖。

Objective To establish the H1299 cell line with HNRNPK downregulated by DOX and study the effect of HNRNPK downregulation on cell proliferation and apoptosis.Methods H1299 cells were infected with a packaged lentivirus.After 72 h,positive cells were selected by puromycin and expanded to a stable transgenic cell line.The expression efficiency of HNRNPK was detected by q PCR and Western blot.The effects of HNRNPK downregulation on cell proliferation and apoptosis were analyzed.Results The H1299 cell line with HNRNPK downregulated by DOX was established successfully.Downregulation of HNRNPK inhibited the proliferation of H1299 cells but did not affect apoptosis.Conclusions Downregulation of HNRNPK inhibits the proliferation of H1299 cells.