摘要
为探讨L14-3菌株对辣椒疫病的生防潜力,采用皿内对峙试验和孢子萌发试验,测定其对辣椒疫霉菌的抑制活性,通过盆栽试验验证其对辣椒疫病的防治效果,并根据形态特征、生理生化特性和16S rDNA序列同源性分析对其进行鉴定。结果表明,L14-3菌株对辣椒疫霉菌的菌丝生长、游动孢子囊的形成、游动孢子的释放和休止孢子的萌发均具有显著的抑制作用;在盆栽试验中,L14-3处理对辣椒疫病的防治效果达到75.00%。经过鉴定,L14-3菌株为多黏类芽孢杆菌(Paenibacillus polymyxa)。
In order to study the biocontrol potential of strain L14-3 against phytophthora blight of pepper,the antifungal activity of strain L14-3 against Phytophthora capsici were detected by in vitro and pot experiments.The results showed that,in vitro experiment,L14-3 was significant inhibit the mycelial growth,zoosporangium production,release and germination of zoospore of P.capsici.In the pot experiment,L14-3 performed the successful potential control against Phytophthora blight of pepper,the control efficiencies was 75.00%.In addition,based on the morphological,physiological characteristics and 16S rDNA sequence analysis,the strain L14-3 was identified as Paenibacillus polymyxa.
作者
潘培培
刘东平
谢太震
张忠良
杜南山
朴凤植
申顺善
PAN Peipei;LIU Dongping;XIE Taizhen;ZHANG Zhongliang;DU Nanshan;PIAO Fengzhi;SHEN Shunshan(Henan New Pesticide Discovery Key Laboratory/College of Plant Protection,Henan Agricultural University,Zhengzhou 450002,Henan,China;College of Horticulture,Henan Agricultural University,Zhengzhou 450002,Henan,China)
出处
《中国瓜菜》
CAS
北大核心
2021年第1期29-34,共6页
China Cucurbits And Vegetables
基金
国家重点研发计划项目(2017YFD0201100)
国家大宗蔬菜产业技术体系建设专项(CARS-23-B03)。
关键词
辣椒
多黏类芽孢杆菌
疫病
抑制活性
防治效果
鉴定
Pepper
Paenibacillus polymyxa
Phytophthora blight
Aantifungal activity
Control effect
Identification
