福州地区0~3岁健康儿童外周血红细胞膜六项脂肪酸指标参考区间调查

Investigation on Reference Intervals of Sixfatty Acids Indexes of Peripheral Blood Erythrocyte Membrane in Healthy Children Aged 0~3 Years in Fuzhou

目的对福州地区0~3岁健康儿童外周血红细胞膜中脂肪酸含量6项指标的参考区间进行调查。方法选取2019年3月~2020年2月来福州儿童医院体检的0~3岁健康儿童60例,分别检测omega-6多不饱和脂肪酸(PUFA)总量(简称omega-6)(包括亚油酸、γ-亚麻酸、二十碳二烯酸、二十碳三烯酸、花生四烯酸和二十二碳四烯酸),omega-3 PUFA总量(简称omega-3)(包括α-亚麻酸、二十碳五烯酸、二十二碳五烯酸和二十二碳六烯酸),单不饱和脂肪酸(MUFA)总量(包括十六碳一烯酸、十八碳一烯酸、二十碳一烯酸和二十四一烯酸),饱和脂肪酸(SFA)总量(包括十四烷酸、十六烷酸、十八烷酸、二十烷酸、二十二烷酸和二十四烷酸),omega-6/omega-3比值和omega-3指数(即二十碳五烯酸与二十二碳六烯酸总和)共6项目,含量以百分比(%)为单位,用于初步调查生物参考区间,均采用x±1.96s表示。结果6项指标不同性别、不同年龄差异均无统计学意义(P>0.05),初步建立omega-6(25.43%~34.09%),omega-3(7.03%~16.68%),MUFA(14.02%~18.70%),SFA(38.95%~46.35%),omega-6/omega-3(1.26~4.61)和omega-3指数(4.62%~13.08%)六项指标参考区间。结论种族、地域、性别、年龄及饮食结构的差异会影响六项目的参考区间,其不仅客观反映了人体内脂肪酸的分布状况,而且为0~3岁健康儿童临床检测的结果提供参照;并为儿童补充omega-3 PUFA提供科学依据,更为进一步建立中国健康人群PUFA的参考区间打下基础。

Objective To investigate the reference intervals of 6 indicators of fatty acid content on erythrocyte membrane in peripheral blood of healthy children aged 0~3 years in Fuzhou.Methods 60 healthy children aged 0~3 years in Fuzhou Children’s Hospital from March 2019 to February 2020 were selected.The fatty acid content on the erythrocyte membrane was measured in percentage(%).The total amount of omega-6 polyunsaturated fatty acids(PUFA,referred to as omega-6,including linoleic acid,γ-linolenic acid,eicosaenoic acid,eicosaenoic acid,arachidonic acid,docosahexaenoic acid),omega-3 PUFA(referred to as omega-3,includingα-linolenic acid,eicosapentaenoic acid,docosahexaenoic acid,docosahexaenoic acid),monounsaturated fatty acids(MUFA,including hexadecanoenoic acid,octadecanoenoic acid,eicosaenoic acid,docosaenoic acid)saturated fatty acids(SFA,including tetradecanoic acid,hexadecanoic acid,octadecanoic acid,icosanoic acid,docosanoic acid,tetradecanoic acid)were detected respectively,and the ratio of 0mega-6/omega-3 and the index of omega-3(EPA+DHA%)were calculated for the initial establishment of the biological reference interval,which were expressed as x±1.96s.Results There were no statistically significant differences in the 6 indicators among children of different genders and ages(P>0.05).Six reference intervals of omega-6(25.43%~34.09%),omega-3(7.03%~16.68%),MUFA(14.02%~18.70%),SFA(38.95%~46.35%),omega-6/omega-3(1.26~4.61)and omega-3 index(4.62%~13.08%)were preliminarily established.Conclusion The differences of race,region,gender,age and diet structure will affect the reference interval of the six items,which not only objectively reflects the distribution of fatty acids in human body,but also provides reference for clinical test results of 0~3 healthy children.It also provides scientific basis for children to supplement omega-3 PUFA,and lays a foundation for further establishing the reference range of PUFA for healthy Chinese population.