脂肪酸合成酶基因单核苷酸多态性与妊娠期糖尿病发病的相关性分析

Association between single nucleotide polymorphism of fatty acid synthetase gene and gestational diabetes mellitus

目的探讨脂肪酸合成酶(FASN)基因单核苷酸多态性(SNP)与妊娠期糖尿病(GDM)发病的相关性。方法选取2016年11月至2019年2月在本院行定期产前检查的450例孕妇作为研究对象,将其分为GDM组(232例,确诊GDM)和对照组(218例,正常妊娠)。通过生物信息学数据库筛选FASN基因的4个候选SNP位点,并通过Sequenom MassARRAY对其进行基因分型。采用多因素Logistic回归模型评估候选SNP位点与GDM发病风险的相关性。结果FASN基因SNP位点rs4485435突变基因型(GC/CC)在GDM组的比例显著低于对照组,差异具有统计学意义(P<0.05)。多因素Logistic回归分析显示,在加性模型下,SNP位点rs4485435基因型与GDM发病风险显著相关,差异具有统计学意义(P<0.05);在显性模型下,携带rs4485435 GC+CC基因型的孕妇GDM发病风险明显低于GG基因型,差异具有统计学意义(P<0.05)。结论FASN基因SNP位点rs4485435多态性与GDM发病风险有关。

Objective To investigate the association between single nucleotide polymorphism(SNP)of fatty acid synthetase gene(FASN)and gestational diabetes mellitus(GDM).Methods A total of 450 pregnant women who underwent regular prenatal examination in our hospital from November 2016 to February 2019 were selected as the research objects and divided into GDM group(232 cases,diagnosed GDM)and control group(218 cases,normal pregnancy).Four candidate SNP of FASN gene were screened by bioinformatics database and genotyped by Sequenom MassARRAY.Multivariate Logistic regression model was used to evaluate the correlation between candidate SNP and the risk of GDM.Results The proportion of rs4485435 mutation genotype(GC/CC)of FASN gene in the GDM group was significantly lower than that in control group,and the difference was statistically significant(P<0.05).The multivariate Logistic regression analysis showed that under the additive model,rs4485435 genotype was significantly associated with the risk of GDM,and the difference was statistically significant(P<0.05);under the dominant model,the risk of GDM of pregnant women with rs4485435 GC+CC genotype was significantly lower than that of GG genotype,and the difference was statistically significant(P<0.05).Conclusion The rs4485435 polymorphism in the SNP locus of FASN gene is related to the risk of GDM.