不同浓度6-BA对变异株云烟97继代培养的影响

Effects of different concentrations of 6-BA on the secondary culture of variant Yunyan 97

在陕西省安康市镇坪县发现2株云烟97巨型变异株,比普通植株有较大的生长优势,利用组培快繁的方式保留这一优良种质资源,以云烟97变异株的腋芽诱导的组培苗为原材料,研究在不同培养基配方下不同6-BA浓度对变异株云烟97继代培养的影响。结果表明,云烟97变异株适宜的分化培养基为二代培养基MS+6-BA 2.0 mg/L,三代培养基MS+6-BA 1.0 mg/L+NAA 0.2 mg/L,在此条件下,变异株分化成芽率较高,达到88.9%.

Two giant variants of Yunyan 97 were found in Zhenping county,Ankang city,Shaanxi province,compared with the com⁃mon tobacco,these giant variants have larger growth advantage.It is a good germplasm resource to be preserved by tissue culture and rapid propagation.The effects of 6-BA concentration on the secondary culture of Yunyan 97 were studied by using tissue culture seed⁃lings induced by axillary buds of Yunyan 97 variant as raw materials.The results showed that the suitable differentiation medium for Yunyan 97 variants was MS+6-BA 2.0 mg/L,and the third generation medium MS+6-BA 1.0 mg/L+NAA 0.2 mg/L,under these con⁃ditions,the differentiation rate of variant was 88.9%.