网络药理学方法研究三七治疗关节炎的作用机制

Mechanism of Panax notoginseng in the treatment of arthritis based on network pharmacology

运用中药系统药理学分析平台(TCMSP)数据库,以化合物口服利用度(OB)>30%、药物相似性(DL)>0.18为条件,检索得到三七(Panax notoginseng)的主要有效成分;通过Drugbank、UniProt数据库进行活性成分靶点提取并用GeneCards、OMIM数据库筛选出关节炎的相关靶点,制作三七治疗关节炎的相关靶点的Veen图,进而使用Cytoscape 3.6.0软件、String数据库分别绘制三七-有效活性成分-靶基因-关节炎的中药网络调控图和蛋白互作(PPI)网络图,最后以R语言为工具,筛选关键靶点并分析其生物过程和重要信号通路,利用网络药理学方法探究三七治疗关节炎的作用机制。结果表明,检索到三七的8个主要成分,得到β-谷甾醇、豆甾醇、人参皂苷rh2等;预测三七治疗关节炎的相关靶点131个;三七关键靶点主要涉及蛋白质异二聚活性、DNA结合转录激活活性,RNA聚合酶II特异性、细胞因子受体结合等生物过程;主要通过糖尿病并发症AGE-RAGE信号通路、流体剪切应力与动脉粥样硬化、乙型肝炎等重要信号通路发挥治疗关节炎的作用。

The main effective components of Panax notoginseng were retrieved by TCMSP database under the condition of OB>30%and DL>0.18;The target of active ingredients was extracted by Drugbank and UniProt database,and the related targets of arthritis were screened by GeneCards and OMIM database;making Veen map of related targets of Panax notoginseng in the treatment of arthri⁃tis;Then,we use the software of Cytoscape 3.6.0 and the database of String to draw PPI network diagram and the network control dia⁃gram of Panax notoginseng,active ingredient,target gene and arthritis respectively;Finally,R language is used as a tool to screen key targets and analyze their biological processes and important signaling pathways,to study the mechanism of Panax notoginseng in the treatment of arthritis by network pharmacology.The results showed that eight main components of Panax notoginseng were re⁃trieved,includingβ-sitosterol,stigmasterol and ginsenoside rh2;131 related targets of Panax notoginseng in the treatment of arthri⁃tis;The key targets of Panax notoginseng are mainly involved in protein heterodimerization activity,DNA-binding transcription acti⁃vator activity,RNA polymerase II-specific,cytokine receptor binding and other biological processes;It is necessary to play a role in the treatment of arthritis through AGE-RAGE signaling pathway in diabetic complication,fluid shear stress and atherosclerosis,Hepa⁃titis B and other important signal pathways.