基于诊断标记的LOX-1活性缺失大麦种质鉴定评价

Diagnostic Marker Based Null LOX-1 Barley Germplasm Identification

在啤酒酿造和储藏过程中,所含脂类物质代谢是影响啤酒风味稳定性和啤酒货架期长短的主要原因。研究表明,大麦脂肪氧化酶1(LOX-1)是导致籽粒中脂类代谢的关键酶,筛选和创制LOX-1活性缺失(null LOX-1)的大麦种质是促进啤酒大麦品质育种的有效途径。为提高检测效率,针对前期鉴定出的中国null LOX-1大麦稀有SNP功能变异,开发出特异性KASP快速诊断标记,并利用该标记对来源于河南和山东两省的633份大麦地方品种进行鉴定评价,共计筛选出8份LOX-1活性缺失新种质,同时明确了该变异的地理分布及其在不同地方品种中的变异频率。本研究不仅为啤酒大麦分子辅助育种提供了优异种质和快速检测手段,也为大麦种质资源遗传完整性保护与利用提供了参考。

The components,which are derived from the lipid metabolism of barley malt,destabilizes the beer flavor and its shelf life in the brewing industry.The lipoxygenase 1(LOX-1)has been approved as the key enzyme involved in lipid metabolism in barley seeds.The identification and development of null LOX-1 barley germplasm might be of interest to promote high-quality malting barley breeding.By taking use of the previously-identified null LOX-1 Chinese barley rare allelic variation,here a Kompetitive Allele Specific PCR(KASP)based diagnostic marker was generated and subjected for a test in 633 barley landraces which originated from Shandong and Henan province.A total of eight null LOX-1 barley accessions were identified,the geographic distribution and the allele frequency were further investigated.Taken together,this work provided LOX-1 free germplasm accessions valuable for the malting barley breeding especially in conjugation with the newly-generated KASP diagnostic marker.Moreover,this study is an example that highlights the importance of the barley genetic integrity in conservation and utilization of germplasm resources.