基于农艺性状指标的山西高粱地方品种核心种质构建

A Core Collection of Sorghum Landraces Formed by Taking Use of Agronomic Traits in Shanxi Province

利用1285份山西省高粱地方品种18个农艺性状的历史数据,通过比较不同取样方法、取样比例和聚类方法组合的构建方法,确定了“多次聚类偏离度取样法+15%取样比例+欧氏距离+最长距离法”为山西省高粱地方核心种质构建的方法。192份初选核心种质和所有样本的均值差异百分率、方差差异百分率、极差符合率和变异系数变化率分别为0、83.33%、97.45%和119.63%。同时,在此基础上补充选择6个具有特殊性状但未选入的种质资源,最终确定198份高粱资源组成山西省高粱地方品种核心种质,取样量为15.4%。经核心种质和所有样本不同性状的均值t测验、极值和标准差比较、遗传多样性指数的t值检验以及主成分分析,最终得出核心种质的198份高粱资源能够代表山西省高粱地方品种的遗传多样性,为山西省高粱地方品种评价和利用提供了优先样本。

This study attempted to build up a core collection of 1285 sorghum germplasm accessions at Shanxi Gene Bank.These accessions were historically investigated for the phenotypic variations 18 agronomic traits,and these datasets were subjected for tests using the combinations on sampling methods,sampling proportion and clustering methods.A pipeline combining multiple cluster deviation sampling method,15%sampling proportion,euclidean distance and complete method was used.By pairwise comparison of phenotypic datasets in core collection(192 accessions)to all samples,the mean difference percentage(MD)was 0,the variance difference percentage(VD)was 83.33%,the coincidence rate of range(CR)was 97.45%,and the variable rate of coefficient of variation(VR)was 119.63%.Furthermore,eight accessions with particular characters were incorporated.This core collection including 198 landraces has been proposed,followed by tests using t-test,extreme value,standard deviation,the t-test of Shannon-Weaver diversity index,as well as principal component.As a result,198 sorghum landraces are representative on the diversity of sorghum landraces hosted by Shanxi Gene Bank,and this collection might be useful in practical uses for sorghum breeding.