肝细胞癌中microRNA的表达水平与早期复发的相关性分析

Correlation between microRNA expression and early recurrence of hepatocellular carcinoma

目的 :探讨肝细胞癌组织中microRNA(miRNA)的表达水平与肝细胞癌早期复发的相关性。方法 :下载公共数据库TCGA的肝细胞癌患者临床信息、随访结果及miRNA表达情况,根据复发时间将患者分为早期复发组(≤2年复发)和晚期复发组(>2年复发),连续变量资料采用独立样本t检验评估2组之间是否存在差异,分类变量资料采用卡方检验评估2组间是否存在差异,将单因素分析P≤0.01的指标纳入logistic多因素回归分析。结果:独立样本t检验分析显示,P≤0.01的miRNA分别为hsa-mir-550a-1、hsa-mir-550a-2、hsa-mir-550a-3、hsa-mir-331、hsa-mir-514a-1、hsa-mir-210、hsa-mir-155、hsa-mir-3170、hsa-mir-4677、hsa-mir-4677、hsa-mir-508、hsa-mir-514a-3、hsa-mir-514a-2、hsa-mir-624,卡方检验显示P≤0.01的指标为患者肿瘤T分期。Logistic多因素回归分析显示hsa-mir-155(OR=0.660,95%CI=0.519~0.841)、hsa-mir-331(OR=1.475,95%CI=1.004~2.168)、hsa-mir-514a-1 (OR=0.603,95%CI=0.413~0.879)、hsa-mir-550a-1 (OR=1.680,95%CI=1.034~2.703)、肿瘤T分期(OR=1.640,95%CI=1.126~2.389)是早期复发的独立影响因素,差异有统计学意义(P<0.05)。结论:肝细胞癌组织中hsa-mir-155、hsa-mir-331、hsa-mir-514a-1、hsa-mir-550a-1的表达水平和肿瘤T分期是影响肝细胞癌术后早期复发的独立影响因素。

Objective:To investigate the correlation between expression of microRNA(m RNA)and early recurrence of hepatocellular carcinoma(HCC).Methods:The clinical information,follow-up results and the expression of miRNAs in TCGA were downloaded from the public database.According to the recurrence time,patients were divided into the early-stage recurrence group(≤2 years recurrence)and the late-stage recurrence group(>2 years recurrence).Independent sample t test was used to assess the difference of continuous variable data in two groups,and chi-square test was used to evaluate the difference of categorical variable data in two groups.Factors(P<0.01)was included in logistic multivariate regression analysis.Results:T-test analysis showed that the miRNA(P≤0.01)was hsa-mir-550 a-1,hsa-mir-550 a-2,hsa-mir-550 a-3,hsa-mir-331,hsa-mir-514 a-1,hsa-mir-210,hsa-mir-155,hsa-mir-mir-3170,hsa-mir-4677,hsa-mir-4677,hsa-mir-508,hsa-mir-514 a-3,hsa-mir-514 a-2 and hsa-mir-624.The chi-square test showed that the indicator(P≤0.01)was tumor T stage.Logistic multivariate regression analysis showed that hsa-mir-155(OR=0.660,95%CI=0.519-0.841),hsa-mir-331(OR=1.475,95%CI=1.004-2.168),hsa-mir-514 a-1(OR=0.603,95%CI=0.413-0.879),hsa-mir-550 a-1(OR=1.680,95%CI=1.034-2.703)and tumor T stage(OR=1.640,95%CI=1.126-2.389)were independent influence factors of early recurrence,with statistically significant differences(P<0.05).Conclusion:Expression levels of hsa-mir-155,hsa-mir-331,hsamir-514 a-1 and hsa-mir-550 a-1 in HCC tissues and tumor T stage are independent factors influencing early recurrence of postoperative HCC.