超高效液相色谱-四极杆/静电场轨道阱高分辨质谱法测定动源性食品中33种兽药残留及质谱库的建立

Detection of veterinary drug residues in animal-derived foods by ultra performance liquid chromatography-high resolution mass spectrometry and establishment of mass library

目的建立超高效液相色谱-四极杆/静电场轨道阱高分辨质谱法测定动源性食品中33种兽药残留的检测方法。方法采用乙腈-水(80:20, V:V)为提取溶剂, QuEChERS方法净化,在Full MS-ddMS2正离子扫描模式下,快速对动源性食品中的目标化合物进行检测。结果在r=70000,扫描范围80~1000m/z内,33种目标化合物的线性相关系数均大于0.99,基质加标回收率范围为70.4%~97.3%,相对标准偏差(relativestandard deviation, RSD)为2.1%~6.0%(n=6)。结论建立的方法适用于动源性食品中33种兽药残留快速检测。同时,本研究建立的33种兽药高分辨质谱库,可应用于非目标化合物的定性筛查。

Objective To establish a method for the determination of 33 veterinary drug residues in animal-derived foods by ultra performance liquid chromatography-high resolution mass spectrometry(UPLC-HRMS). Methods The samples were extract with acetonitrile-water(80:20, V:V), and purified by QuEChERS. In the Full MS ddMS~2 positive ion scanning mode, the target compounds in animal-derived foods food were detected quickly. Results Under the conditions of Full MS(80~1000 m/z) and 70000 mass resolution, the 33 veterinary drug residues had good linear relationships(r≥0.99). The matrix recoveries were between 70.4%-97.3%. The relative standard deviations were between 2.1%-6.0%(n=6). Conclusion The established method is suitable for rapid detection and analysis of 33 veterinary drug residues in animal-derived foods. An accurate mass library has been established and the library can be used to screen and confirm the non-target compounds.