CRH通过CRHR1激活cAMP/PKA通路调节宫颈癌细胞的免疫逃逸和促进宫颈癌生长

CRH activates cAMP/PKA pathway via CRHR1 to regulate the immune escape of cervical cancer cells and promote the growth of cervical cancer

目的探讨促肾上腺皮质激素释放激素(corticotropin-releasing hormone,CRH)及其受体(corticotropinreleasing hormone receptor 1,CRHR1)影响宫颈癌免疫逃逸和生长的途径。方法采用实时荧光定量聚合酶链反应(quantitative real-time PCR,RT-qPCR)和Western blot检测宫颈癌组织、宫颈癌细胞(HeLa细胞)和人宫颈永生化上皮细胞(H8细胞)中CRH、CRHR1、CTLA-4、IL-10和TGF-β1的表达量;用不同浓度CRH(0、1、10及20 nmol/L)作用细胞48 h或用10 nmol/L CRH作用细胞不同时间点后(12、24、36及48 h),通过CCK-8法检测各组细胞的增殖能力;CRH、CRH+CRHR1选择性阻断剂安塔拉明(CRH+Antalarmin)分别作用细胞后,RT-qPCR检测细胞CTLA-4的表达量,Western blot检测细胞中cAMP、PKA蛋白的磷酸化水平和Bax、Bcl-2、IL-10和TGF-β1表达量,CCK-8法测定细胞增殖能力;CRH、CRH+PKA通路抑制剂H-89(CRH+H-89)分别作用细胞后,RT-qPCR检测细胞CTLA-4的表达量,Western blot检测细胞中cAMP、PKA蛋白的磷酸化水平和IL-10、TGF-β1、Bax和Bcl-2表达量,CCK-8法测定细胞增殖能力。结果与癌旁正常组织和H8细胞相比,宫颈癌组织和HeLa细胞中的CRH及CRHR1表达量均显著升高(P<0.01);1、10及20 nmol/L CRH作用48 h后细胞增殖能力明显高于0 nmol/L CRH处理组(P<0.01);10 nmol/L CRH作用细胞24、36和48 h后细胞增殖能力明显高于CRH作用12 h组(P<0.01)。CRH通过CRHR1激活cAMP/PKA信号通路,促进细胞的增殖以及CTLA-4、IL-10、TGF-β1的表达。和Control组相比,CRH组细胞的CTLA-4、Bcl-2、IL-10和TGF-β1表达量升高,Bax表达量明显降低;和CRH组相比,CRH+H-89组细胞的CTLA-4、Bcl-2、IL-10和TGF-β1表达量降低,Bax表达量明显升高。结论CRH通过受体CRHR1促进宫颈癌的免疫逃逸和生长,其作用机制可能与激活cAMP/PKA通路有关。

The main purpose of this study was to investigate the effect of corticotropin-releasing hormone(CRH)and its corticotropin-releasing hormone receptor 1(CRHR1)on the immune escape and growth of cervical cancer.RT-qPCR and Western blot were used to detect the expression levels of CRH,CRHR1 CTLA-4,IL-10 and TGF-β1 in cervical cancer tissues,HeLa and H8 cells.The proliferative activity of cells in each group was detected by CCK-8 after the cells were treated with different concentrations of CRH for 48 h or affter the cells were treated with 10 nmol/L CRH at different time points.Western blot was used to detected the expression of CTLA-4,the phosphorylation levels of cAMP and PKA proteins and the expression lelvels of Bax,Bcl-2,IL-10 and TGF-β1 in the cells treated with CRH,CRH+Antalarmin or CRH+H-89.Date showed that the expression levels of CRH and CRHR1 in cervical cancer tissues and cells were significantly higher than those in para-carcinoma normal tissues and H8 cells.The proliferative capacity of cells treated with 1,10 and 20 nmol/L CRH for 48 h was significantly higher than that of cells treated with 0 nmol/L CRH.The proliferative ability of cells treated with 10 nmol/L CRH for 24 h,36 h and 48 h was significantly higher than that of cells treated for 12 h.CRH activated the cAMP/PKA signaling pathway through CRHR1,thus promoted cell proliferation,and increased the expression of CTLA-4,IL-10,TGF-β1.Furthermore,the expression levels of CTLA-4,Bcl-2,IL-10 and TGF-β1 were increased,and the expression of Bax was decreased in CRH treated cells;the expression lelves of CTLA-4,Bcl-2,IL-10 and TGF-β1 were decreased and the expression of Bax was increased in CRH+H-89 treated cells.Taken together,CRH promotes immune escape and growth of cervical cancer through the receptor CRHR1,and its mechanism may related to the activation of cAMP/PKA pathway.