卵黄高磷蛋白调控生物玻璃对牙本质的再矿化

Regulation of Phosvitin on Remineralization of Demineralized Dentin by Bioactive Glass

目的:探讨卵黄高磷蛋白(PV)调控生物活性玻璃对脱矿牙本质再矿化的能力。方法:制备120片牙本质切片,随机分为6组,A组为对照组(SBF模拟体液),B组为100 mg/L PV,C组为500 mg/L PV,D组为SBF+生物活性玻璃(BG),E组为100 mg/L PV+BG组,F组为500 mg/L PV+BG组。模拟口腔温度处理30 d后,扫描电镜(SEM)观察牙本质表面的显微结构并测量表面显微硬度值(SMH)。结果:E组和F组与其余各组SMH值均有统计学差异(P<0.01),但两组间无显著差异(P>0.05),C组和D组与A组比较有统计学差异(P<0.05)。SEM观察显示各组均有沉积物形成,E组和F组沉积物更加均匀致密。结论:卵黄高磷蛋白(PV)可以促进牙本质表面矿物质的形成,与BG联合应用可有加强协同作用。

Objective:To investigate the regulation of phosvitin(PV)on remineralization of demineralized dentin by bioactive glass(BG).Methods:120 dentin slices were prepared and randomly divided into 6 groups:Group A was treated with SBF as the control group,Group B was treated with 100 mg/L PV,Group C was treated with 500 mg/L PV,Group D was treated with SBF and BG,Group E was treated with 100 mg/L PV and BG,and Group F was treated with 500 mg/L PV and BG.After simulated oral temperature treatment for 30 days,the microstructure of dentin surface was observed by scanning electron microscope(SEM)and the values of surface microhardness(SMH)was measured.Results:The SMH values of group E and F were significantly different from those of other groups(P<0.01),but there was no significant difference between two groups(P>0.05).SMH value of group C or D was significantly different from that of group A(P<0.05).Remineralization were observed in all groups,and the sediments of group E and F were more uniform and tighter.Conclusion:PV can promote the remineralization on the dentin surface,and the combination of PV and BG achieves the synergistic effect.