VacA基因与口腔鳞癌组织中VEGF表达的相关性分析

Analysis of correlation between VacA gene and VEGF expression in oral squamous cell carcinoma

目的:检测口腔鳞状细胞癌组织中是否存在幽门螺杆菌毒力因子VacA基因以及与VEGF表达的相关性,探讨幽门螺杆菌与口腔鳞状细胞癌的关系。方法:采用聚合酶链反应检测40例口腔鳞状细胞癌组织和23例正常黏膜组织中VacA基因的阳性率,采用免疫组化染色检测VEGF的表达情况,并比较口腔鳞状细胞癌组织VacA基因阳性组和阴性组中VEGF表达的差异。结果:OSCC组织中VacA基因阳性率为25%,正常口腔黏膜VacA基因阳性率为0,两者差异有统计学意义(P=0.024)。VacA阳性组OSCC组织中VEGF的强阳性率高于VacA阴性组(P=0.018),VacA阳性组中VEGF的平均光密度高于VacA阴性组(P=0.023),差异均有统计学意义。结论:OSCC中存在VacA基因,并与VEGF的表达可能存在相关性,幽门螺杆菌是否通过VacA毒力因子调控VEGF的表达而影响OSCC的发生和发展,需要进一步研究。

Objective:To detect the presence of Helicobacter pylori virulence factor VacA gene and its correlation with VEGF expression in oral squamous cell carcinoma(OSCC)tissues,and explore the relationship between Helicobacter pylori and oral squamous cell carcinoma.Methods:The polymerase chain reaction(PCR)was used to detect the positive rate of VacA gene in 40 cases of oral squamous cell carcinoma tissues and 23 normal mucosal tissues,and the immunohistochemical staining was used to detect the expression of VEGF.The difference in VEGF expression between VacA gene positive and negative groups in oral cancer tissues was compared.Results:The positive rate of VacA gene was 25%in OSCC and 0 in normal oral mucosa,and the difference was statistically significant(P=0.024).The strong positive rate of VEGF in OSCC of VacA positive group was higher than that of VacA negative group(P=0.018),and the average optical density of VEGF in VacA positive group was higher than that of VacA negative group(P=0.023).The difference was statistically significant.Conclusion:There is VacA gene in OSCC,and it may be related to VEGF expression.Whether Helicobacter pylori affects the occurrence and development of OSCC through the regulation of VEGF expression by VacA virulence factors should be further studied.