基于网络药理学探讨黄芪散干预骨质疏松的分子作用机制

Discussion on the molecular mechanism of Huangqi San on osteoporosis based on network pharmacology

目的:运用网络药理学及其相关方法探究黄芪散干预骨质疏松(Osteoporosis,OP)的相关分子作用机制。方法:首先依托各相关数据库筛选黄芪散及OP相关靶点,然后将两组靶点映射出共同作用靶点。根据自由度筛选出关键靶点,最后将关键靶点输入基因功能注释数据库(DAVID数据库)进行富集分析。结果:黄芪散经数据库筛选并去重整合后获得活性成分15个,靶点151个,OP相关靶点336个,映射出共同作用靶点20个。黄芪散干预骨质疏松可能通过AKT1、SRC、ESR1、PGR、AR等关键靶点调控前列腺生长、雄激素代谢过程、子宫发育等生物过程及雌激素、Rap1、促性腺激素释放激素(GnRH)、甲状腺激素、前列腺癌等多条信号通路。结论:应用网络药理学技术方法可揭示黄芪散治疗骨质疏松的潜在作用机制,具有较高的置信度和参考价值,黄芪散能通过多个靶点调控多条与OP相关通路,多方面、多层次干预OP进程。

Objective: To explore the molecular mechanism of Huangqi San( 黄芪散) in the intervention of OP by using network pharmacology and related methods. Methods: Firstly, the related targets of Huangqi San and OP were screened based on the relevant databases, and then the targets of two groups were mapped to the common target. According to the degree of freedom, the key targets were selected, and finally the key targets were input into DAVID database for enrichment analysis. Results: 15 active ingredients, 151 targets, 336 OP-related targets and 20 co-acting targets were obtained after screening and de-integration by database. The intervention of Huangqi San on osteoporosis may regulate prostate growth, androgen metabolism, uterine development and other biological processes, as well as estrogen, Rap1, GnRH, thyroid hormone, prostate cancer and other signal pathways through key targets such as AKT1, SRC, ESR1, PGR and AR. Conclusion: The application of network pharmacology technology can reveal the potential mechanism of the Huangqi San in the treatment of osteoporosis, which has high confidence and reference value. The Huangqi San can regulate multiple OP-related pathways through multiple targets, and intervene in the OP process in various ways and at multiple levels.