不同干姜炮制品姜酚类成分含量的研究

A quantitative analysis of the different contents of gingerols in different processed zingiberis rhizoma

目的:对干姜不同炮制品中6-姜酚、8-姜酚、10-姜酚、6-姜烯酚4种姜酚类成分进行含量测定,比较姜酚类成分含量的差异,为临床用药提供实验依据。方法:采用反相高效液相色谱法,使用Agilent TC-C18柱(5μm,4.6 mm×250 mm),流动相为乙腈-水溶液,梯度洗脱;流速:1.0 mL/min;检测波长:280 nm;柱温:30℃,进样量20μL。结果:干姜不同炮制品中4个成分的色谱峰均达到基线分离,不同炮制方法中姜酚类成分量含量依次为:干姜生品>土炒干姜>清炒干姜>姜炭>炮姜,其中6-姜烯酚的含量差异,依含量高低依次为:干姜生品>炮姜>姜炭>土炒干姜>清炒干姜。结论:本含量测定方法能够准确测定干姜不同炮制品姜酚类的含量,可为干姜不同炮制品的质量控制提供研究方法;不同干姜炮制品中姜酚类成分含量存在差异。

Objective: To determine 6-gingerol, 8-gingerol, 10-gingerol, 6-shogaol in zingiberis rhizoma with different processing methods and compare the different contents of gingerols, to provide experimental basis for clinical medication. Methods: Samples were analyzed on an Agilent TC-C18 column(5μm, 4.6 mm×250 mm) with the mobile phase of acetonitrile and water in a gradient elution. The flow rate of the mobile phase, detection wavelength, column temperature and injection volumn were set at 1.0 mL/min, 280 nm, 30 ℃ and 20 μL, respectively. Results: The chromatographic peaks of the 4 components in zingiberis rhizoma with different processing methods were all separated at baseline.The contents of gingerols in different processing methods were as follows by decreasing order: zingiberis rhizoma, soil-processed zingiberis rhizoma, stir-fried zingiberis rhizoma, carbon ginger, zingiberis rhizoma praeparatum. The contents of 6-shogaol were as follows by decreasing order: zingiberis rhizoma, zingiberis rhizoma praeparatum, carbon ginger, soil-processed zingiberis rhizoma, stir-fried zingiberis rhizoma. Conclusion: The method can be used to determine the contents of gingerols in different processed zingiberis rhizoma and can be used for the quality control of different processed zingiberis rhizoma. The contents of gingerols in different zingiberis rhizoma processed products were different.