树突状细胞上巨噬细胞半乳糖型凝集素在乳腺癌细胞识别中的作用研究

Effect study of MGL on dendritic cells in breast cancer cell recognition

目的:探索乳腺癌细胞系MCF-7与巨噬细胞半乳糖型凝集素(MGL)的结合情况,并对MCF-7细胞表面的MGL配体进行鉴定。方法:采用流式细胞术和单糖抑制实验检测MCF-7细胞表面糖抗原的GalNAc表达以及MGL与MCF-7细胞的结合情况。RT-PCR、Western blot和免疫沉淀等研究方法对MCF-7上MGL配体进行鉴定。结果:MGL能够与乳腺癌细胞MCF-7高度结合,且这种结合是Ca2+依赖的;乳腺癌细胞MCF-7表面高表达GalNAc,即Tn抗原;单糖GalNAc的加入可明显抑制MGL与乳腺癌细胞MCF-7的相互作用;黏蛋白MUC1为乳腺癌细胞系MCF-7表面MGL的配体。结论:黏蛋白MUC1为乳腺癌细胞系MCF-7表面MGL的配体,介导了MGL对乳腺癌细胞的免疫识别。

Objective:To investigate the binding of breast cancer cell line MCF-7 with macrophage galactose lectin(MGL),and to identify the MGL ligand on the surface of MCF-7 cell.Methods:Flow cytometry and monosaccharide inhibition test were used to detect the expression of GalNAc on the surface of MCF-7 cells,and the binding of MGL to MCF-7 cells.The MGL ligands on MCF-7 were identified by RT-PCR,Western blot and immunoprecipitation.Results:MGL could highly bind to MCF-7 cells,and the binding was Ca2+-dependent;the surface of MCF-7 cells highly expressed GalNAc,namely Tn antigen.The addition of monosaccharide GalNAc could significantly inhibit the interaction between MGL and MCF-7.Mucin MUC1 was the ligand of MGL on MCF-7.Conclusion:MUC1 is a ligand of MGL on the surface of MCF-7 cell line and mediates the immune recognition of breast cancer cells by MGL.