基于ZnO纳米刷的展青霉素电化学适配体传感器的构建与应用

Construction and application of electrochemical aptasensor based on brush-shaped nano-ZnO for patulin detection

建立了一种基于刷状纳米级ZnO富集,适配体特异性识别的电化学传感技术用于果品中展青霉素(patulin,PAT)的高灵敏度检测。ZnO纳米刷的引入可以有效增大PAT特异性适配体的负载量,进而增强对待测体系中目标物PAT的识别和捕获能力,实现PAT高灵敏度检测。结果表明,该方法检测PAT的线性范围是0.5~1×10^3μg/L,最低检出限为0.15μg/L,检测苹果汁和山楂片样品的平均加标回收率为94.75%~110.84%,对于赭曲霉毒素A具有较好的抗干扰能力。该方法结合可控形貌纳米材料富集了PAT适配体的生物识别能力,从而实现了对PAT的快速、准确、高灵敏度检测,为其他真菌毒素传感器的构建提供了一个新的思路。

In this paper,an electrochemical sensor technology based on brush-shaped nano-ZnO enrichment and aptamer specific recognition was established for high-sensitivity detection of patulin(PAT)in fruits.It was introduced to increase the load of PAT-specific aptamers,thereby enhancing the identification and capture capabilities of this sensing system for the target PAT,and realizing high-sensitivity detection of PAT.Results showed that the linear range of this method was 0.5-1×10^3μg/L with the detection limit of 0.15μg/L.And the average recovery of spiked apple juice and hawthorn tablets was between 94.75%and 110.84%which has a good anti-interference ability for ochratoxin A.This method combined with the controllable morphology nanomaterials enriched the biorecognition ability of PAT aptamers,thereby could realize the rapid,accurate and high sensitivity detection of PAT,and provide a new idea for the construction of other mycotoxin sensors.