款冬叶片总黄酮提取工艺优化及抗氧化活性研究

Optimization of Extraction Method and Antioxidant Activities of Total Flavonid From the Leaves of Tussilago farfara L.

以款冬叶片为试材,采用微波辅助法提取总黄酮,在单因素试验的基础上,采用正交实验法优化款冬叶片总黄酮提取工艺,同时以分光光度法测定提取物对1,1-二苯基-2-三硝基苯肼自由基(1,1-diphenyl-2-picrylhydrazyl,DPPH·)、羟基自由基(·OH)和超氧阴离子自由基(O-·2)的清除作用。结果表明:款冬叶片总黄酮最佳提取条件为乙醇体积分数70%,微波提取时间3 min,微波功率500 W,料液比1∶15 g·mL^-1,在该条件下,总黄酮的提取率为0.91%。款冬叶片总黄酮的质量浓度与抗氧化活性呈一定的线性关系,当总黄酮质量浓度为0.069 8 mg·mL^-1时,对DPPH·、·OH和O-·2的清除率分别达84.21%、84.10%和68.32%,表明款冬叶片具有良好的抗氧化能力,可作为天然的抗氧化剂进一步的开发和利用。

The leaves of Tussilago farfara L.were used as test materials,the total flavonoid of which was extracted by microwave-assisted method on the basis of the results of single factor experiment in order to optimize the total flavonoid from the leaves of T.farfara through the orthogonal experiment.Meanwhile,the scavenging effect of extraction on 1,1-diphenyl-2-picrylhydrazyl radical(DPPH·),hydroxyl free radical(·OH) and superoxide anion free radical(O-·2) were measured and determined by spectrophotometry method.The results showed that the optimum extraction parameters were ethanol volume fraction was 70%,microwave extraction time was 3 minutes,ratio of solid to liquid was 1∶15 g·mL^-1,microwave power was 500 W.Under these conditions,the extraction rate of total flavonoid from T.farara leaves was 0.91%.In addition,there was a linear relationship between the mass concentration and the antioxidant activity.The clearance rate could be reached to 84.21%,84.10% and 68.32% corresponding to DPPH·,·OH and O-·2,respectively,with the concentration of total flavonoids as 0.069 8 mg·mL^-1.Therefore,the total flavonoids from the leaves of T.farfara could have the outstanding antioxidant ability and maybe developed and utilized deeply as the natural antioxidants in the future.