两种化学型广藿香叶显微特征比较

Comparative on Leaf Microcharacteristics of Two Chemotypes of Pogostemon cablin

以6个不同产地的2种化学型广藿香叶片为试材,采用数码显微系统对叶片的多种显微特征进行观察比较,采用SPSS 21.0、MEGA 7、SIMCA 14.1软件进行脉岛数、栅表比、气孔指数、非腺毛数、头状腺毛数、盾状腺毛数显微常数的方差分析、聚类分析和主成分分析试验,以期为广藿香叶片显微特征与化学型分型的研究及广藿香品质评价提供参考依据。结果表明:2种化学型广藿香叶表皮细胞形状、表皮细胞长宽比、气孔大小及类型相近;叶缘、叶长宽比、垂周壁形状、表皮细胞大小、栅表比、脉岛数、气孔指数、非腺毛数、头状腺毛数、盾状腺毛数差异显著,可以作为区分广藿香不同化学型的评判指标;四会、高州、雷州、阳春4个产地的广藿香归为一类,均为醇型,莲塘、龙洞产地归为一类,均为酮型。

Taking Pogostemon cablin leaves from six origins with identified chemotypes as test materials.A digital microscope system was used to observe and compare various microscopic characteristics of P.cablin leaves.Variance analysis,cluster analysis and principal component analysis were performed on microconstants,such as leaf vein islandsnumber,palisade ratio,stomatal index,non-glandular hair number,capitate trichomes number and peltate trichomes number using SPSS 21.0,MEGA 7 and SIMCA 14.1.This study could provide reference for the study of the relationship between the microscopic characteristics of P.cablin leaves and the chemotype classification and the quality evaluation of P.cablin.The results showed that the two chemotypes of P.cablin leaf were similar in epidermal cell shape,epidermal cell aspect ratio,stomata size and type.There were significant differences in leaf margin,leaf aspect ratio,the pattern of vertical wall,epidermal cell size,palisade ratio,leaf vein islands number,stomatal index,non-glandular hair number,capitate trichomes number and peltate trichomes number,which could be used as evaluation index for distinguishing different chemotypes of P.cablin.P.cablin from Sihui,Gaozhou,Leizhou and Yangchun city were classified as patchoulol-type,while those from Liantang and Longdong were classified as pogostone-type.