蝴蝶兰切花花被不同开放阶段酶活性的变化

Changes in Enzymatic Activity of Different Flowering Stages of Phalaenopsis aphrodite Cut-flower

以蝴蝶兰鲜切花为试材,对切花花被不同生理阶段3种酶活性的变化进行测量分析,以期为蝴蝶兰切花寿命的延长提供参考依据。结果表明:萼片和花瓣中的过氧化物酶(POD)从花苞期到半开期酶活性变化不明显,从半开期到衰老期酶活性显著升高。花瓣中的超氧化物歧化酶(SOD)从花苞期到半开期酶活性升高,从半开期到衰老期酶活性显著降低;萼片中的超氧化物歧化酶活性变化不显著但也呈先上升后下降趋势。花瓣中的中性蛋白酶(NP)从花苞期到半开期酶活性呈上升趋势,半开期到盛开期呈下降趋势,盛开期到衰老期呈上升趋势;花萼中的中性蛋白酶从花苞期到盛开期酶活性呈下降趋势,从盛开期到衰老期呈上升趋势;总体来看中性蛋白酶的活性呈先下降后上升趋势。

Taking fresh cut-flower of Phalaenopsis aphrodite as test materials,the changes in the activities of the three enzymes at different stages of the cut-flower tepals were measured and analyzed,in order to provide reference for prolonging the life span cut-flower of Phalaenopsis aphrodite.The results showed that the enzyme activity of peroxidase in sepals and petals did not change significantly from the bud stage to the half-blooming stage,and the enzyme activity increased significantly from the half-blooming stage to the senescence stage.The enzyme activity of superoxide dismutase in petals increased from the bud stage to the half-blooming stage,and the enzyme activity decreased significantly from the half-blooming stage to the senescence stage.The enzyme activity of neutral protease in the petals shows an upward trend from the bud stage to the half-blooming stage,a downward trend from the half-blooming stage to the blooming stage,and an upward trend from the blooming stage to the senescence stage.However,In the sepals the enzyme activity of the neutral protease showed a downward trend from the bud stage to the blooming stage,and an upward trend from the blooming stage to the senescence stage.Overall,the enzyme activity of neutral protease in both sepals and petals showed a first decline and then an upward trend.