猪苓多糖对胃肠上皮化生细胞模型的影响

Effect of polyporus umbellatus polysaccharides on gastrointestinal metaplasia cell model induced in vitro

目的:探讨猪苓多糖对鹅去氧胆酸(CDCA)诱导GES-1细胞株的肠上皮化生相关分子标志物的影响。方法:CCK8法检测不同浓度CDCA、猪苓多糖对GES-1细胞增殖能力的影响;RT-qPCR、Western blot法检测肠上皮化生相关分子标志物表达水平。结果:CDCA诱导后,GES-1细胞的尾型同源盒2(CDX2)、Kruppel样转录因子4(KLF4)蛋白及mRNA表达水平明显上调(P<0.05),性别决定区Y框蛋白2(SOX2)蛋白及mRNA表达水平显著下调(P<0.05)。猪苓多糖干预后可抑制由CDCA诱导的肠上皮化生细胞模型CDX2、KLF4的mRNA和蛋白表达(P<0.05),并促进SOX2 mRNA和蛋白的表达(P<0.05)。结论:猪苓多糖可通过下调CDX2和KLF4的表达,上调SOX2的表达,从而在一定程度上阻断胃黏膜上皮细胞向肠上皮化生转变的进程。

Objective:To explore the effect of polyporus umbellatus polysaccharides on gastrointestinal metaplasia of GES-1 cells induced by chenodeoxycholic acid.Methods:The effects of chenodeoxycholic acid and Polyporus umbellatus polysaccharide at different concentrations on the proliferation of GES-1 cells was detected by CCK8,and the expression of intestinal metaplasia related molecular markers was determined by RT-qPCR and Western blot.Results:After GES-1 cells were induced by chenodeoxycholic acid,the expression levels of CDX2,KLF4 protein and mRNA were significantly up-regulated(P<0.05),while the expression levels of SOX2 protein and mRNA were significantly down-regulated(P<0.05).Polyporus umbellatus polysaccharides can inhibit the expression of mRNA and protein of CDX2 and KLF4 in intestinal metaplasia cell model induced by CDCA(P<0.05),and promote the expression of SOX2 mRNA and protein(P<0.05).Conclusion:Polyporus umbellatus polysaccharides can block the transformation of gastric mucosal epithelial cells to intestinal metaplasia to some extent by down-regulating the expression of CDX2 and KLF4 and up-regulating the expression of SOX2.