陆地棉细胞色素P450超家族基因GhCYP85A2-1的克隆与功能分析

Cloning and Functional Analysis of Cytochrome P450 Superfamily Gene GhCYP85A2-1 in Upland Cotton

【目的】研究细胞色素P450超家族CYP85A亚家族基因在棉花株高发育中的生物学功能,为陆地棉株高分子育种提供理论依据和基因资源。【方法】采用同源克隆方法,从陆地棉中克隆CYP85A家族基因GhCYP85A2-1,利用烟草脆裂病毒(Tobacco rattle virus,TRV)诱导的基因沉默技术(Virus-induced gene silencing,VIGS)构建GhCYP85A2-1基因沉默载体,利用qRT-PCR技术检测侵染棉花幼苗的基因表达量。【结果】GhCYP85A2-1基因沉默植株的株高显著降低,基因的表达量也显著下降。【结论】GhCYP85A2-1基因在棉花株高发育过程中起到重要调控作用。

【Objective】The principal objective is to investigate the biological function of CYP85A subfamily genes of the cytochrome P450 superfamily in the development of cotton plant height in the hope of providing a scientific basis for molecular breeding of upland cotton plants.【Method】A CYP85A family gene GhCYP85A2-1 was cloned from upland cotton by homologous cloning.Tobacco rattle virus(TRV)induced gene silencing technology(Virus-induced gene silencing,VIGS)was used to construct GhCYP85A2-1 gene silencing vector,total RNA was extracted,and gene expression of GhCYP85A2-1 in infecting cotton seedlings was investigated by qRT-PCR.【Result】The study found that the height of GhCYP85A2-1 gene silenced plants was significantly reduced,and so was the gene expression.【Conclusion】GhCYP85A2-1 gene plays an important regulatory role in the development of cotton plant height.