饲粮中不同棉秆比例对绵羊瘤胃发酵参数和血清生化指标的影响

Effects of Cotton Stalk Ratios in Diets on Rumen Fermentation Parameters and Serum Biochemical Indexes of Sheep

【目的】研究不同比例棉秆对绵羊瘤胃发酵参数和血清生化指标的影响。【方法】试验采用单因素完全随机试验设计,将体重相近的50只试验羊随机分为5组,每组10只。对照组为不含棉秆的基础饲粮(CK组),试验组饲粮分别为以10%(A组)、20%(B组)、30%(C组)和40%(D组)棉秆替代基础饲粮中的精料和粗料。【结果】(1)瘤胃液中氨态氮浓度随棉秆替代比例增加而下降,D组比CK组显著降低42.02%(P<0.05),其它各组间差异不显著(P>0.05)。瘤胃pH值、乙酸、丙酸、丁酸和总挥发性脂肪酸含量组间差异不显著(P>0.05)。(2)血清中碱性磷酸酶含量,D组比CK组降低32.14%(P<0.05)。尿素氮和血糖含量组间无差异(P>0.05)。【结论】饲粮中棉秆比例为10%~40%对绵羊瘤胃挥发性脂肪酸含量无影响,当比例达到40%时对瘤胃氨态氮浓度和血清碱性磷酸酶含量影响较大。

【Objective】This experiment is conducted to investigate the effects of different ratios of cotton stalk in diets on rumen fermentation parameters and serum biochemical indexes of sheep.【Method】Fifty sheep with the similar body weight were randomly assigned to five groups with ten sheep in each group by a single factor completely randomized design.Sheep in control group were fed a basal diet,and sheep in trial groups were fed the diets whose roughage in basal diets were replaced by 10%(group A),20%(group B),30%(group C)and 40%(group D)cotton stalks,respectively.【Result】The results showed as follows:(1)The rumen ammonia concentration was decreased with the increasing replacement ration of cotton stalk,group D was significant lower than group CK by 42.02%(P<0.05),and no significant effects were observed among the other groups;no significant effects on pH,acetate,propionate,butyrate and TVFA concentration were found(P>0.05).(2)As the replacement ration of cotton stalk in diets increased,the serum ALP content in group D was significant lower than that in group CK by 32.14%(P<0.05),and there were no significant differences in serum urea nitrogen and glucose content in 5 groups(P>0.05).【Conclusion】Under this experimental condition,there are no significant effects on VFA content of rumen fluid when cotton stalk ratio in diets were 10%-40%.When cotton stalk ratio in diet is 40%,the contents of rumen ammonia nitrogen and serum ALP change obviously.