外源油菜素内酯对毛竹实生苗生理特性的影响

Effects of exogenous BR on physiological characteristics of Phyllostachys edulis seedlings

【目的】研究外源喷施油菜素内酯对毛竹Phyllostachys edulis生理特性的影响,为未来毛竹种植栽培及管理提供理论依据和技术参考。【方法】以毛竹实生苗为试材,通过水培试验,设置6种不同质量浓度油菜素内酯溶液处理[0(对照)、0.0500、0.0100、0.0050、0.0010、0.0005 mg·L^−1],比较处理间毛竹实生苗生长性状、光合色素、光合特性以及抗氧化系统等的差异。【结果】从整体上看,与对照相比,不同质量浓度的油菜素内酯在不同程度上对毛竹实生苗生长发育,对光合色素质量分数及光合能力起到提升作用。相较于对照,当油菜素内酯质量浓度为0.0050和0.0010 mg·L^−1时,毛竹实生苗超氧化物歧化酶活性分别提高6.7%和6.1%,过氧化物酶活性分别提高14.6%和13.7%,过氧化氢酶活性分别提高8.2%和14.6%,可溶性蛋白质质量分数分别增加31.5%和31.2%,淀粉质量分数分别增加17.3%和16.9%;而质量浓度为0.0500、0.0100及0.0005 mg·L^−1的油菜素内酯处理与0.0050和0.0010 mg·L^−1质量浓度处理相比,对毛竹实生苗抗氧化性能与渗透调节能力的提升效果并不明显。【结论】油菜素内酯能提高毛竹实生苗光合能力,增强抗逆境能力,从而促进其营养生长,且当质量浓度在0.0050~0.0010 mg·L^−1时效果最好。

[Objective]This research aims to study the effects of exogenous brassinolide(BR)on physiological characteristics of Phyllostachys edulis,and to provide theoretical basis and technical reference for future cultivation and management of Ph.edulis.[Method]The seedlings of Ph.edulis were used as test materials,and 6 kinds of brassinolide solutions with different concentrations[0(ck),0.0500,0.0100,0.0050,0.0010,0.0005 mg·L^−1]were set up through hydroponic culture experiment to compare the differences in the growth characteristics,photosynthetic pigment,photosynthetic characteristics and antioxidant system of the seedlings.[Result]On the whole,compared with ck,brassinolide with different mass concentrations could promote the growth and development of Ph.edulis seedlings to varying degrees,improve the quality of photosynthetic pigments and photosynthetic capacity,and promote the synthesis of osmotic adjustment substances.Compared with ck,when the mass concentration of brassinolide was 0.0050 and 0.0010 mg·L^−1,the superoxide dismutase activity of bamboo seedlings increased by 6.7%and 6.1%,respectively,oxidase activity increased by 14.6%and 13.7%,and catalase activity increased by 8.2%and 14.6%,respectively.The mass fraction of soluble protein increased by 31.5%and 31.2%,and the mass fraction of starch increased by 17.3%and 16.9%,respectively.However,compared with 0.0050 and 0.0010 mg·L^−1 treatment,brassinolide treated with mass concentration of 0.0500,0.0100 and 0.0005 mg·L^−1 had no significant effect on antioxidant capacity and osmotic adjustment ability of Ph.edulis seedlings.[Conclusion]Brassinolide can improve the photosynthetic capacity and stress resistance of Ph.edulis seedlings,and promote nutrient growth.The best concentration of brassinolide is 0.0050−0.0010 mg·L^−1.