摘要
Objective:To study the effects of the novel vanillin derivative BVAN08 on the radiosensitivity of hepatic cancer cells for the purpose of providing evidence for its potential use as a potential radiosensitive drug.Methods:Hepatic cancer Huh-7 cells labeled with luciferase were used to investigate the radiosensitivity induced by BVAN08 in vivo.Colony formation assays and flow cytometry were used to measure the apoptosis and radiosensitivity of Huh-7 cells in vitro produced by BVAN08.Histology and immunohistochemistry were used to evaluate the toxicity of BVAN08 in vivo.Results:BVAN08 induced apoptosis and cell cycle arrest of Huh-7 cells in a time-dependent manner.Moreover,BVAN08 combined with radiation increased the sensitivity of Huh-7 cells toγ-ray radiation and significantly inhibited tumor growth in vivo.The tumor inhibition rates of the BVAN08 treatment group,irradiation treatment group,and combined therapy group were 58%,38%,and 85%,respectively.The DNA-PKcs expression in tumor tissues of the BVAN08 treatment group was lower than that of the control group(P<0.01).BVAN08 inhibited the growth of Huh-7 cells in nude mice bearing tumors,without resulting in any noticeable side effects on bodyweight,livers,hearts,kidneys,or the number of peripheral white blood cells.Conclusions:As a natural food additive derivative,BVAN08 possesses the potential to be used as an effective anticancer drug to increase cell sensitivity to radiotherapy.
基金
This study was supported by the National Natural Science Foundation of China(grant Nos.:31870847 and 11705283)and the foundation of Jinzhou Medical School。
