FoxO3a/p27在创伤性脑损伤中的表达变化及意义

THE EXPRESSION AND SIGNIFICANCE OF FOXO3A/P27 IN TRAUMATIC BRAIN INJURY

目的探索中枢神经系统损伤和修复的机制。方法制作大鼠TBI模型,取其脑组织进行免疫印迹分析和免疫组织化学,观察其中FoxO3a与p27的表达。结果 FoxO3a表达量在伤后3 d显著下调,p27水平在伤后1 d显著下降,二者表达呈正相关(r=0.902,P=0.002);FoxO3a染色在损伤3 d后的对侧大脑中表达广泛,而在损伤侧大脑中染色水平较低。结论 FoxO3a和p27极有可能通过调控细胞周期参与神经系统损伤后修复过程。

Objective To explore the mechanism of central nervous system injury and repair.Methods TBI rat models were established,and the expression of FoxO3a and p27 in brain tissue was observed by Western blot analysis and immunohistochemistry.Results FoxO3a expression was significantly down-regulated on day 3 after injury,and p27 level was significantly down-regulated on day 1 after injury,with a positive correlation(r=0.902,P=0.002).FoxO3a staining was widely expressed in the contralateral brain after 3 days of injury,while the staining level was low in the injured brain.Conclusion FoxO3a and p27 may be involved in the repair process after nervous system injury by regulating the cell cycle.