异甘草素对颅脑损伤大鼠IL-6和IL-10表达的影响

Effect of isoliquiritigenin on the expression of IL-6 and IL-10 factor in rats after traumatic brain injury

目的探讨异甘草素(ISL)对大鼠颅脑创伤(TBI)后炎性因子白介素-6(IL-6)和抗炎因子IL-10表达的影响及TBI后脑保护作用。方法Feeney法将75只雄性Wistar大鼠制作TBI模型,按随机数字表法分为空白对照组(5只)、TBI组(35只)及ISL干预组(35只)。酶联免疫吸附试验法分别测定TBI后以及TBI经ISL干预后3、12、24、48、72 h及5、7 d各时间点IL-6及IL-10的变化,同时用干湿重法测量各时间点脑组织含水量变化,找出其相关性及内在联系。结果3组大鼠的IL-6及IL-10表达水平比较,差异均有统计学意义(P<0.05),经各时间点测定比较,IL-6及IL-10在TBI组及ISL干预组均较对照组均有所上升。TBI经ISL干预后,ISL干预组IL-6的表达水平在各个时间点较TBI组下降,IL-10的表达水平在各个时间点较TBI组升高,差异均有统计学意义(P<0.05)。TBI后脑组织含水量有所增加,经ISL干预后脑组织含水量在72 h及5 d下降明显,差异有统计学意义(P<0.05)。经Pearson相关分析,TBI组随着IL-6的升高,IL-10亦相应升高,呈正相关(r=0.925,P=0.000);ISL干预组IL-6升高而IL-10升高,呈正相关(r=0.888,P=0.000)。结论ISL可通过抑制TBI后炎性因子过度表达及促进抗炎因子表达水平,减轻TBI后炎性反应,减轻脑水肿而发挥脑保护作用。

Objective To investigate the effect of isoliquiritigenin(ISL)on the expression of inflammatory factor interleukin-6(IL-6)and anti-inflammatory factor IL-10 after traumatic brain injury(TBI)in rats,and the protective effect of ISL on brain after TBI.Methods Seventy five male Wistar rats were randomly divided into blank control group(n=5),TBI group(n=35)and ISL intervention group(n=35)according to the random table,after making TBI model by Feeney method.The enzyme-linked immunosorbent assay method was used to measure the changes of IL-6 and IL-10 at each time point of 3,12,24,48,72 h,5 and 7 d after TBI and after TBI intervention by ISL,and colleagues used dry and wet weight method to measure the changes in brain tissue water content,find out its correlation and internal connections.Results The expression levels of IL-6 and IL-10 in TBI group and ISL intervention group were significantly higher than those in control group(P<0.05).After intervention with ISL,the expression level of IL-6 in TBI group was lower than that in TBI group at each time point,while the expression level of IL-10 in TBI group was higher than that in TBI group at each time point,and the differences were statistically significant(P<0.05).The water content of brain tissue increased after TBI,and decreased significantly at 72 h and 5 d after ISL intervention(P<0.05).Pearson correlation analysis showed that with the increase of IL-6,the level of IL-10 in TBI group was also increased,there is a positive correlation between them(r=0.925,P=0.000);IL-6 was increased and IL-10 was increased in ISL intervention group,there is a positive correlation between them(r=0.888,P=0.000).Conclusion ISL can protect the brain by inhibiting the over expression of inflammatory factors and promoting the expression of anti-inflammatory factors after TBI,reducing the inflammatory reaction and brain edema after TBI.