利用植物叶片鉴定耐盐性的简便方法探索

Exploring a simple method to identify salt tolerance in plant leaves

盐胁迫是影响植物生长和分布的重要非生物胁迫因素,简便快速鉴定耐盐种质资源对盐碱地的改良和利用具有重要意义。该研究选取河北省沧州市黄骅市南大港盐碱地自然生长的25种植物为研究对象,测定其叶片SPAD值、相对电导率、含水量、Na^+含量和K^+含量,通过统计方法筛选简便鉴定测定植物耐盐性。结果表明:相对电导率与SPAD值、Na^+含量和K^+含量均呈极显著的负相关关系;含水量与Na^+含量呈极显著的正相关关系,与K^+含量呈显著正相关关系;Na^+含量与K^+含量呈极显著的正相关关系;其他指标相互之间均无显著的相关关系。因此,可以通过以测定叶片相对电导率为主,SPAD值和Na^+含量为辅,分析植物的耐盐性强弱,为快速鉴定耐盐植物提供了选择的方法。

Salt stress is an important abiotic stress factor affecting plant growth and distribution. It is of great significance to identify salt-tolerant germplasm resources quickly and easily for the improvement and utilization of saline-alkali land. In this study, 25 plants naturally growing in Nandagang saline-alkali land in Cangzhou City, Hebei Province were selected as the research objects, and the SPAD value, relative electrical conductivity, water content, Na^+ content and K^+ content of their leaves were measured, and the salt tolerance of plants was simply identified and determined by statistical methods. The results showed that the relative conductivity was negatively correlated with SPAD value, Na^+ content and K^+ content. There was a significant positive correlation between water content and Na^+ content and K^+ content. The content of Na^+ was positively correlated with that of K^+. Other indexes had no significant correlation with each other. Therefore, the relative electrical conductivity of leaves can be determined as the main method, and the SPAD value and Na^+ content can be used as the auxiliary method to analyze the salinity tolerance of plants, which provides a selective method for rapid identification of salt tolerant plants.