母乳低聚糖组成及其对益生菌的增殖作用

Oligosaccharide Composition of Human Breast Milk and Its Effect on Promoting the Proliferation of Probiotics

研究混合母乳中复杂的母乳低聚糖(human milk oligosaccharides,HMOs)组成及含量,选择双歧杆菌Bb-12、发酵乳杆菌CECT57162种益生菌,研究HMOs 2’-岩藻糖(2’-fucosyllactose,2’-FL)对2种益生菌的增殖作用。结果表明:2个混合样本的HMOs含量分别为10.613、5.093 g/L,差异很大,说明不同母乳来源的HMOs受诸多因素影响,但含量范围一般为5~15 g/L;2’-FL对发酵乳杆菌CECT5716和双歧杆菌Bb-12均有一定的增殖效果,说明益生菌可以利用HMOs进行生长和增殖。

The composition and contents of oligosaccharides in two mixed samples of human breast milk were determined and the effect of the human milk oligosaccharides(HMOs)2’-fucosyllactose(2’-FL)on the proliferation of two probiotic strains,Bifidobacterium Bb-12 and Lactobacillus fermentum CECT5716 was evaluated in this study.The results showed that the HMOs contents of the two mixed samples were 10.613 and 5.093 g/L respectively.This significant difference indicated that HMOs contents in breast milk from different mothers are affected by many factors,generally ranging between 5 and 15 g/L.2’-FL promoted the growth of both priobiotics,indicating that the probiotics could utilize HMOs for growth and proliferation.