气相液氮罐内温度差异对脐带血造血干细胞保存质量的影响

Effect of Temperature Difference in Gas Phase Liquid Nitrogen Tank on the Preservation Quality of Umbilicalcord Blood Hematopoietic Stem Cells

目的研究气相液氮罐内因位置不同而导致的温度差异对脐带血造血干细胞保存质量的影响。方法选取位于液氮中的脐血干细胞20份作为对照组A组,保存在气相液氮罐内最低温度保存处的20份样本为实验B组,最高温度保存处的20组样品为实验C组。各组细胞解冻复苏后进行有核细胞计数、有核细胞活性、CD34^+%、CFU-GM等检测,进行单因素方差分析。结果三组细胞的有核细胞回收率、有核细胞活性变化率、CFU-GM回收率及CD34^+细胞回收率差异无统计学意义(P>0.05)。结论在不高于–188℃的温度范围内(液氮罐内标本保存的最高位置),因高度引起的温度差异并不会对脐带血造血干细胞的冻存质量产生影响。

Objective To study the effect of temperature difference caused by different storage location ingas phase liquid nitrogen tank on the cryopreservation quality of umbilical cord blood(UCB)derived hematopoietic stem cells.Methods Twenty units of UCB hematopoietic cells in liquid nitrogen were selected as control group A,twenty units of samples preserved at the lowest temperature in the gas phase liquid nitrogen tank as experimental group B,and twenty units preserved at the highest temperature in the tank as experimental group C.After thawing and resuscitation,nucleated cell count,cell activity,CD34^+%and CFU-GM were detected and one-way ANOVA was performed.Results There was no significant difference in the recovery rate of nucleated cells,the change rate of activity of nucleated cells,the recovery rate of CFU-GM and the recovery rate of CD34^+cells among the three groups.(P>0.05)Conclusion The temperature difference caused by storage position has no effect on the cryopreservation quality of UCB hematopoietic stem cells in the temperature range of no more than–188℃.