新辅助化疗对ⅠB~ⅡB期宫颈癌病灶内恶性生物学特征的影响

Effects of neoadjuvant chemotherapy on malignant biological characteristics inⅠB~ⅡB stage cervical cancer lesions

目的探讨新辅助化疗对ⅠB~ⅡB期宫颈癌病灶内恶性生物学特征的影响。方法选取2015年4月至2018年1月间在洪湖市人民医院接受治疗的ⅠB~ⅡB期宫颈癌病人157例,均接受术前新辅助化疗+根治性手术治疗。留取新辅助化疗前后宫颈癌组织标本各157份作为研究对象。对比新辅助化疗前后,宫颈癌组织中增殖、侵袭、自噬相关基因表达量的差异。结果新辅助化疗后,宫颈癌组织中增殖相关基因FOXP3[(50.18±5.64)比(77.49±8.12)]、HOXA7[(59.26±7.18)比(86.34±9.73)]、USP22[(75.09±8.62)比(101.26±13.18)]、Sox2mRNA[(67.91±8.65)比(95.42±11.83)]的表达量低于新辅助化疗前,TSLC1mRNA的表达量高于新辅助化疗前[(125.48±14.19)比(90.72±9.82)];侵袭相关基因FAK[(69.33±8.51)比(102.17±14.58)]、MACC1[(71.09±8.56)比(93.22±10.81)]、GRP94[(65.88±9.21)比(90.71±9.85)]、PRPS2[(89.07±9.23)比(114.32±15.68)]、TPX2 mRNA[(71.71±8.38)比(85.36±9.21)]的表达量低于新辅助化疗前;自噬相关基因Beclin1[(131.75±15.59)比(106.38±14.32)]、LC3-B[(120.66±13.58)比(94.27±11.60)]、PI3KC3mRNA[(100.64±12.38)比(85.48±9.02)]的表达量均高于新辅助化疗前。结论ⅠB~ⅡB期宫颈癌病人在根治性手术前接受新辅助化疗,可进一步抑制癌细胞的增殖、侵袭活力并调节其自噬活性。

Objective To investigate the effects of neoadjuvant chemotherapy on malignant biological characteristics inⅠB-ⅡB stage cervical cancer lesions.Methods Totally 157 patients withⅠB-ⅡB stage cervical cancer who were treated in The People’s Hospital of Honghu between April 2015 and January 2018 were selected,and all of them underwent preoperative neoadjuvant chemotherapy plus radical operation.A hundred and fifty-seven cervical cancer tissue samples were collected before and after neoadjuvant chemotherapy respectively.The differences in the expression of genes related to proliferation,invasion and autophagy in cervical cancer tissues were compared before and after neoadjuvant chemotherapy.Results After neoadjuvant chemotherapy,proliferation-related genes FOXP3[(50.18±5.64)vs.(77.49±8.12)],HOXA7[(59.26±7.18)vs.(86.34±9.73)],USP22[(75.09±8.62)vs.(101.26±13.18)]and Sox2 mRNA[(67.91±8.65)vs.(95.42±11.83)]expressions in cervical cancer tissues were lower than those before neoadjuvant chemotherapy whereas TSLC1 mRNA expression was higher than that before neoadjuvant chemotherapy[(125.48±14.19)vs.(90.72±9.82)].Invasion-related genes FAK[(69.33±8.51)vs.(102.17±14.58)],MACC1[(71.09±8.56)vs.(93.22±10.81)],GRP94[(65.88±9.21)vs.(90.71±9.85)],PRPS2[(89.07±9.23)vs.(114.32±15.68)],TPX2 mRNA[(71.71±8.38)vs.(85.36±9.21)]expressions were lower than those before neoadjuvant chemotherapy.Autophagy-related genes Beclinl[(131.75±15.59)vs.(106.38±14.32)],LC3-B[(12.0.66±13.58)vs.(94.2.7±11.60)],PI3 KC3 mRNA[(100.64±12.38)vs.(85.48±9.02)]expressions were higher than those before neoadjuvant chemotherapy.Conclusion Neoadjuvant chemotherapy before radical operation can further inhibit the proliferation and invasion activity of cancer cells and adjust their autophagy activity in patients withⅠB-ⅡB stage cervical cancer.