幽门螺杆菌感染病儿胃黏膜组织微小RNA-497-3p表达及与Toll样受体8/Toll样受体10/核因子κB信号通路的相关性

Expression of miR-497-3p in gastric mucosal tissues of children patients with helicobacterpylori infection and its correlation with TLR8/TLR10/NF-κB signal pathway

目的观察微小RNA(miR)-497-3p在幽门螺杆菌(Hp)感染病儿胃黏膜组织中表达水平,并探讨其与核因子(NF)-κB信号通路的相关性。方法选取2014年1月至2018年11月黄冈市中心医院就诊的病儿210例为研究对象,分为Hp感染组(n=100)和Hp非感染组(n=110)。实时荧光定量聚合酶链式反应(qRT-PCR)检测miR-497-3p、Toll样受体8(TLR8)、Toll样受体10(TLR10)和NF-κB mRNA的表达情况,酶联免疫吸附测定(ELISA)检测病儿胃黏膜组织中肿瘤坏死因子(TNF-α)、白细胞介素-6(IL-6)、白细胞介素-8(IL-8)和白细胞介素-10(IL-10)水平。结果Hp感染组的胃黏膜组织中miR-497-3p mRNA相对表达量显著低于Hp非感染组[(1.53±0.16)比(2.46±0.14)],TLR8[(1.84±0.15)比(1.05±0.23)]、TLR10[(1.92±0.06)比(1.38±0.09)]和NF-κB[(2.56±0.03)比(1.40±0.02)]相对表达量均高于Hp非感染组,差异有统计学意义(均P<0.05);Hp感染组的胃黏膜组织中TNF-α[(21.18±1.35)比(11.50±2.37)]、IL-6[(1.74±0.15)比(1.05±0.23)]、IL-8[(1.92±0.06)比(1.38±0.09)]和IL-10[(2.56±0.03)比(1.40±0.02)]的表达水平均高于Hp非感染组,均差异有统计学意义;Pearson相关分析显示,Hp感染组病儿胃黏膜组织中miR-497-3p与TLR8、TLR10和NF-κB mRNA相对表达量、TNF-α、IL-6、IL-8、IL-10水平均呈负相关(均P<0.05)。miRNA-497-3p诊断Hp感染的ROC曲线下面积(AUC)为0.926,根据SPSS统计结果计算miR-497-3p在最佳临界值处诊断Hp敏感度和特异度分别为91.8%和93.7%。结论miR-497-3p在Hp感染组的胃黏膜组织中异常低表达,其可能通过影响TLR8/TLR10/NF-κB信号通路介导的炎性反应,从而在Hp感染的致病过程中发挥作用,为临床下一步的治疗提供思路。

Objective To investigate the expression level of microRNA(miR)-497-3p in gastric mucosa of children infected with Helicobacter pylori(Hp),and to explore its correlation with nuclear factor(NF)-κB signaling pathway.MethodsA total of 210 cases treated in Huanggang Central Hospital from January 2014 to November 2018 were selected and assigned into Hp infection group(n=100)and Hp non-infection group(n=110).Real-time fluorescent quantitative PCR(qRT-PCR)was used to detect the expressions of miR-497-3p,TLR8,TLR10 and NF-κB mRNA,and enzyme-linked immunosorbent assay(ELISA)was used to detect the levels of tumor necrosis factor(TNF-α),interleukin-6(IL-6),IL-8 and IL-10 in gastric mucosa tissue of the children.ResultsIn Hp infection group,the relative expression of miR-497-3p in gastric mucosa tissue was significantly lower than that in Hp non-infection group[(1.53±0.16)vs.(2.46±0.14)],and the relative expressions of TLR8[(1.84±0.15)vs.(1.05±0.23)],TLR10[(1.92±0.06)vs.(1.38±0.09)]and NF-κB[(2.56±0.03)vs.(1.40±0.02)]were higher than those in the Hp non-infection group;the differences were statistically significant(all P<0.05).The expression levels of TNF-α[(21.18±1.35)vs.(11.50±2.37)],IL-6[(1.74±0.15)vs.(1.05±0.23)],IL-8[(1.92±0.06)vs.(1.38±0.09)]and IL-10[(2.56±0.03)vs.(1.40±0.02)]in gastric mucosa tissue were higher in HP infection group than in Hp non-infection group,with statistically significant differences.The Pearson correlation analysis showed that the expression of miR-497-3p in gastric mucosa tissue in HP infection group was negatively correlated with the relative expression levels of TLR8,TLR10,NF-κB mRNA,and the relative expression levels of TNF-α,IL-6,IL-8 and IL-10(all P<0.05).Area under the ROC curve(AUC)of miRNA-497-3p in the diagnosis of Hp infection was 0.926.The sensitivity and specificity of miR-497-3p at the optimal critical value were 91.8%and 93.7%on the basis of the statistical results of SPSS.ConclusionThe abnormally low level of miR-497-3p in gastric mucosa tissue of children in HP infection group may play a role in the process of HP infection by inflammatory response affecting the TLR8/TLR10/NF-κB signaling pathway mediation,which provides clinical ideas for further treatment.