具有癌变潜能胃炎的炎性细胞表型特征及其差异表达基因富集通路分析

Phenotypic Characteristics and Differentially Expressed Genes Enrichment Pathway of Inflammatory Cells in Gastritis With Cancerous Potential

背景:胃癌,特别是肠型胃癌是由非萎缩性胃炎-萎缩性胃炎-异型增生-癌变的过程发展而来。目的:探讨易于癌变的胃炎(萎缩性胃炎)和不易于癌变的胃炎(非萎缩性胃炎)的炎性细胞表型特征及其差异表达基因富集的通路。方法:从GEO数据库中下载GSE2669、GSE83389、GSE106656和GSE27411数据集,采用R语言筛选差异表达基因,并采用GSE116312数据集进行验证。三个“非萎缩性胃炎-萎缩性胃炎”数据集筛选出的差异表达基因与炎性细胞表型特征基因取交集,筛选出的差异表达基因行REACTOME和KEGG分析。结果:在“正常胃黏膜-非萎缩性胃炎-萎缩性胃炎”的疾病动态链中,筛选出多种差异表达基因,以GSE116312数据验证后得到5个共同差异表达基因。3个数据集共筛选出85个炎性细胞表型特征基因,在“正常胃黏膜-非萎缩性胃炎”疾病链中中性粒细胞比例较高,在“非萎缩性胃炎-萎缩性胃炎”疾病链中为成纤维细胞和巨噬细胞。REACTOME和KEGG分析发现,“非萎缩性胃炎-萎缩性胃炎”疾病链中,差异表达基因富集于IL-10、IL-4和IL-13信号通路以及抗原处理与呈递信号通路。结论:具有癌变潜能胃炎的炎性细胞表型特征多为巨噬细胞、中性粒细胞和成纤维细胞,多富集于IL信号通路以及抗原处理与呈递信号通路。

Background:Gastric cancer,especially intestinal-type gastric cancer,is considered as the result of the process of non-atrophic gastritis-atrophic gastritis-dysplasia-carcinogenesis.Aims:To explore the phenotypic characteristics and differentially expressed genes(DEGs)enrichment pathway of inflammatory cells between gastritis that prone to canceration(atrophic gastritis)and gastritis that not prone to canceration(non-atrophic gastritis).Methods:The datasets of GSE2669,GSE83389,GSE106656 and GSE27411 were downloaded from GEO database.DEGs were screened by R language and verified by GSE116312 dataset.DEGs screened from 3 datasets of‘non-atrophic gastritis-atrophic gastritis’were overlapped with inflammatory cell phenotypic characteristic genes.REACTOME and KEGG analyses of DEGs were performed.Results:A variety of DEGs in the‘normal gastric mucosa-non-atrophic gastritis-atrophic gastritis’dynamic process were screened,and 5 common genes were verified by GSE116312 dataset.A total of 85 inflammatory cell phenotypic characteristic genes were screened from 3 datasets.The percentage of neutrophil was high during‘normal gastric mucosa-non-atrophic gastritis’process while the percentages of fibroblast and macrophage were high during‘non-atrophic gastritis-atrophic gastritis’process.REACTOME and KEGG analyses showed that DEGs of inflammatory cell phenotype during‘non-atrophic gastritis-atrophic gastritis’process were mainly enriched in IL-10,IL-4 and IL-13 signaling pathways and antigen presentation pathway.Conclusions:Macrophage,neutrophil and fibroblast are the inflammatory cell phenotypic characteristics of gastritis with cancerous potential,which enriched in IL and antigen presentation pathways.