高血压及高血压合并糖尿病的患者血浆可溶性NRP-1与SOD浓度的变化

Change of Plasma Level of Soluble NRP-1 and SOD in Hypertensive Patients and Hyperten⁃sive Diabetic Patients

【目的】探讨高血压和高血压合并糖尿病患者血浆可溶性神经纤毛蛋白-1(NRP-1)的浓度与超氧化物歧化酶(SOD)活性的变化。【方法】本横断面研究共纳入88人,分为对照组(n=26)、高血压组(n=31)和高血压合并糖尿病组(n=31)。人群血浆NRP-1的浓度和SOD活性应用酶联免疫吸附法测定,同时检测血糖、糖化血红蛋白A1C(GHbA1c)和血脂的变化情况。【结果】单纯高血压组血清总胆固醇(TC)和体质量指数(BMI)显著高于对照组(P<0.05),而高血压合并糖尿病组血清TC、低密度脂蛋白胆固醇(LDL-C)、甘油三酯(TG)、BMI和腰围(WC)均显著高于对照组(P<0.05)。单纯高血压组和高血压合并糖尿病组血浆NRP-1的浓度和SOD活性均低于对照组[NRP-1(ng/mL):6.8(6.0~8.3)、5.2(4.0~6.8)vs 8.9(7.7~10.0);SOD(U/mL):157.1±18.6、145.1±31.4 vs 168.4±23.1,P<0.05],而高血压合并糖尿病组与单纯高血压组比较血浆NRP-1的浓度和SOD活性更低,且差异也有统计学意义[NRP-1(ng/mL):5.2(4.0~6.8)vs 6.8(6.0~8.3);SOD(U/mL):145.1±31.4 vs 157.1±18.6,P<0.05]。对3组研究人群分别进行线性相关性分析发现,NRP-1与SOD之间均呈显著正相关(r=0.539,0.660,0.895,P<0.05)。【结论】单纯高血压和高血压合并糖尿病患者血浆NRP-1的浓度均降低,而且高血压合并糖尿病患者血浆NRP-1的浓度降低更明显,NRP-1的浓度下降可能与SOD活性的下降有关。

【Objective】To explore the changes of plasma levels of soluble neuropilin-1 and superoxide dismutase(SOD)in hypertensive patients and hypertensive diabetic patients.【Methods】In this cross-sectional study,eighty-eight cases were enrolled,which were divided into hypertensive group(n=31),hypertensive diabetic group(n=31)and control group(n=26).The levels of neuropilin-1 and SODwere measured by ELISA.Meanwhile,the levels of serum glucose,glycosylated hemoglobin A1C(GHbA1c)and lipid profile were detected.【Results】The levels of total cholesterol(TC)and body mass index(BMI)were significantly higher in the hypertensive group than those in control group(P<0.05).The levels of TC,low density lipoprotein cholesterol(LDL-C),triglyceride(TG),BMI,waist circumference were significantly higher in the hypertensive diabetic group than those in control group(P<0.05).The mean plasma levels of neuropilin-1 and SOD in both hypertensive diabetic group and hypertensive group were significantly decreased compared with that in the normal group[NRP-1(ng/mL):6.8(6.0~8.3),5.2(4.0~6.8)vs 8.9(7.7~10.0);SOD(U/mL):157.1±18.6,145.1±31.4 vs 168.4±23.1,P<0.05]While the mean plasma levels of neuropilin-1 and SOD in hypertensive diabetic group were significantly decreased compared withthat of the hypertensive group[NRP-1(ng/mL):5.2(4.0~6.8)vs 6.8(6.0~8.3);SOD(U/mL):145.1±31.4 vs 157.1±18.6,P<0.05].The linear correlation analysis of three groups of study population found that neuropilin-1 and SOD showed significant positive correlation(r=0.539,0.660,0.895,P<0.05).【Conclusions】The plasma concentration of neuropilin-1 is decreased in both hypertensive and hypertensive diabetic patients,and more decreased in hypertensive diabetic patients.Decreased neuropilin-1 concentration may be associated with the reduction of SOD activity.