红细胞膜表面黏附分子与红细胞寿命的相关性研究

Correlation between adhesion molecules on the surface of red blood cell membrane and red blood cell lifespan

目的探讨红细胞寿命与其膜表面蛋白黏附分子的关系,以期建立1种检测红细胞贮存时间的方法。方法采集10人(份)健康无偿献血者的新鲜红细胞标本10 mL/份,应用Percoll密度梯度离心法将红细胞分成5个年龄(层),流式检测术检测各分层红细胞膜表面黏附分子CD47、CD44、CD147的表达量;应用SPSS统计软件对分层的红细胞蛋白表达量做方差分析。结果年轻至年老各层红细胞膜表面3种黏附分子表达量(%)依次为CD47:14.44±2.61,9.30±1.75,7.84±1.49,6.54±1.32,5.53±1.12(P<0.01);CD44:25.01±1.94,19.22±1.52,17.10±1.28,15.18±1.11,13.56±1.08(P<0.01);CD147:33.46±1.99,28.31±2.95,23.83±1.59,20.40±1.56,18.03±1.65(P<0.01)。结论随着红细胞寿命的增长,红细胞膜表面CD47、CD44、CD147表达水平呈下降趋势,这3种蛋白黏附分子或与红细胞寿命有关,可作为提示红细胞寿命的检测标志物。

Objective To explore the correlation between red blood cell lifespan and adhesion molecules on the surface of red blood cell membrane, in order to establish a method to detect the duration of red blood cell storage. Methods 10 samples(10 mL each) of fresh red blood cell, collectedf rom 10 healthy voluntary blood donors, were divided into 5 age groups(layers) by Percoll density gradient centrifugation. The expression of CD47, CD44 and CD147 on the surface of red blood cell membrane in each layer was detected using flow cytometry. The variance of protein expression in each layer of red blood cells was analyzed by SPSS statistical software. Results The expression levels(%) of 3 adhesion molecules on the surface of red blood cell membranes from young to old were CD47: 14.44±2.61, 9.30±1.75, 7.84±1.49, 6.54±1.32 and 5.53±1.12(P<0.01);CD44: 25.01±1.94, 19.22±1.52, 17.10±1.28, 15.18±1.11 and 13.56±1.08(P<0.01);CD147: 33.46±1.99, 28.31±2.95, 23.83±1.59, 20.40±1.56 and 18.03±1.65(P<0.01). Conclusion The expression levels of CD47, CD44 and CD147 on the surface of red blood cell membranes have showed a downward trend as the storage extended. These three protein adhesion molecules have showed a correlation with red blood cells lifespan, and could be used as detection markers of cell age.