马铃薯杂种后代性状评价及SSR指纹分析

Evaluations on Traits and SSR Fingerprint Analysis of Potato Hybrid Progenies

为了选育抗晚疫病、高产、优质的马铃薯新品种,本试验以CIP引进晚疫病抗性强的材料388676.1为母本,以克新23为父本进行杂交,获得杂种F1群体,并利用SSR分子标记技术对优良株系进行DNA鉴定。结果表明:结合植株形态、单株产量、淀粉含量等农艺性状进行评价,选育出综合性状优异的品系7和品系8。利用筛选出的2对SSR特异性引物,对9个品系及其亲本的基因组DNA进行扩增,建立了新品系的SSR指纹图谱,可进一步促进马铃薯品种快速鉴定。

In order to breed new potato varieties with high resistance to late blight,high yield and high quality,the potato material CIP388676.1 with strong late blight resistance was used as female parent,and the variety Kexin 23 was used as male parent,we obtained hybrids F1 by crossing,and the SSR molecular marker technology was used to identify the F1 superior individuals.The results showed that combined with evaluation of agronomic traits including plant morphology,tuber yield and starch content,we finally selected two strains with excellent comprehensive traits.The SSR fingerprint of nine potato strains and their parents was established by using two SSR special primers to amplify genome DNA.The research results could promote rapid identification of potato.