灰葡萄孢基因组果胶酶生物信息学分析

Bioinformatics Analysis of Pectinase in Botrytis cinerea Genome

为有效防控果蔬灰霉病,本研究采用生物信息学方法对灰葡萄孢基因组内果胶酶的分布情况、理化性质及三级结构进行了分析。结果表明:灰葡萄孢基因组内检索出6条PG氨基酸序列,4条PME蛋白氨基酸序列和4个PEL家族蛋白。BcPG1~BcPG16蛋白序列长度为370~514 aa,信号肽长度在16~22 aa,磷酸化位点丰富;BcPME1~BcPME14蛋白序列长度为327~348 aa,信号肽长度在18~23 aa,都不存在N-糖基化位点,磷酸化位点多;BcPEL1~BcPEL4蛋白序列长度为236~353 aa,信号肽长度在17~20 aa,存在多个O-糖基化位点,磷酸化位点多。3种果胶酶氨基酸序列分别存在保守区域,三级结构预测结果显示同一家族存在相似结构域,进化分析表明灰葡萄孢中的3种果胶酶具有保守性。

In order to control the gray mold effectively,the distribution,physical and chemical properties,and tertiary structure of pectinase in Botrytis cinerea genome were analyzed by bioinformatics in this study.The results showed that there were 6 PG,4 PME and 4 PEL in B.cinerea genome.PG sequences were 370-514 aa in length with 16-21 aa signal peptides and abundant phosphorylation sites.PME sequences were 327-348 aa in length with 18-23 aa signal peptides and many phosphorylation sites,but no N-glycation sites.While PEL protein sequences were 236-353 aa in length,with 17-20 aa signal peptides,and abundant in O-glycations sites and phosphorylation sites.Conservative regions existed three kinds of pectinase amino acid sequence respectively.Tertiary structure prediction results showed that similar domain structure presented in the same family.Evolutionary analysis indicated that pectinases were conserved in B.cinerea genome.