摘要
为了鉴定荔枝酸腐病的病原,利用组织分离法从新鲜发病的荔枝果实组织中分离并纯化出培养物,再经单孢分离获得3个菌株LCG-1、LCG-2和LCG-3;经形态学特征和rDNA-ITS序列分析,从而对分离菌株进行鉴定。结果表明:3个菌株形态学和分子生物学特性一致,综合鉴定为白地霉(Geotrichum candidum Link&Magazin)。根据柯赫氏法则对3个菌株进行致病性测定,接种果实的致病率均为100%,而对照无症状表现,因而证实分离菌株为荔枝酸腐病的病原菌。
In order to confirm the pathogen of litchi acid rot,three isolates,LCG-1,LCG-2,and LCG-3,were isolated and purified from fresh litchi fruit tissues by tissue isolation method,and identified by morphological characteristics and the rDNA-ITS sequences analysis.The results showed that the morphological and molecular biological characteristics of the three strains were consistent,and they were identified as Geotrichum candidum Link&Magazin.According to Koch's rule,the pathogenicity of the three strains were tested.The results showed that the incidence rates of all inoculated fruits were 100%,while the control showed no symptoms.Therefore,the isolates were identified as the pathogen causing litchi sour rot.
作者
黄国林
刘月廉
朱艳秋
HUANG Guo-lin;LIU Yue-lian;ZHU Yan-qiu(Zhanjiang Lyuhai Bioengineering Limited Company,Zhanjiang 524200,China;Binhai Agricultural College,Guangdong Ocean University,Zhanjiang 524088,China)
出处
《黑龙江农业科学》
2021年第2期66-69,共4页
Heilongjiang Agricultural Sciences
基金
广东海洋大学大学生创新创业训练计划项目(CXXL2019177)
湛江市科技项目(2016B01038)。
关键词
荔枝
酸腐病
白地霉
鉴定
Litchi chinensis
sour rot
Geotrichum candidum
identification
