肝爽颗粒对肝硬化和抑郁症“异病同治”的网络药理学机制分析

Analysis on the mechanisms of treating different diseases with same method of Ganshuang Granule in treating cirrhosis with depression based on network pharmacology

[目的]通过网络药理学分析肝硬化和抑郁症的共同发病机制。构建肝爽颗粒中药-成分-靶点网络、蛋白互作网络,初步探索肝爽颗粒“异病同治”肝硬化和抑郁症的共同作用机制。[方法]通过OMIM和Drugbank数据库获取肝硬化和抑郁症相关靶点,结合TCMID、TCMSP和Batman-TCM数据库及相关技术平台挖掘肝爽颗粒化学成分和作用靶点。使用Ingenuity Pathway Analysis(IPA)软件,寻找肝硬化和抑郁症靶点相关的共同通路和功能。将肝爽颗粒治疗两种疾病的共同作用靶点导入BioGPS和Genecards数据库中获得组织和亚细胞分布,并进行通路和功能注释分析,绘制疾病治疗网络。[结果]肝硬化和抑郁症的共同潜在发病机制可能与免疫功能失调(树突细胞成熟、Th1和Th2活化信号通路、白细胞迁移)、炎症反应(急性期反应信号、糖皮质激素受体信号、HMGB1信号、器官炎症反应)和糖脂代谢异常(肝脏胆汁淤积、葡萄糖代谢障碍、糖尿病)相关。肝爽颗粒可能通过免疫调控、抗炎和调节糖脂代谢作用治疗两种疾病。[结论]肝硬化和抑郁症共同发病机制涉及免疫功能失调、炎症反应和糖脂代谢异常。肝爽颗粒可能通过调节免疫功能和糖脂代谢治疗两种疾病,为深入阐述肝爽颗粒治疗作用及药理机制提供方向。

[Objective]The common pathogenesis of cirrhosis and depression were analyzed through the network pharmacology,and the interaction network of Ganshuang Granules’traditional Chinese medicine-component-target network was constructed,so as to preliminarily explore the common pathogenesis of cirrhosis and depression and explore the mechanism of Ganshuang Granules’effect of treating two different diseases.[Methods]OMIM and Drugbank database were the targets related to cirrhosis and depression,combined with TCMID,TCMSP and Batman-TCM database and literature mining to obtain the ingredients and targets of Ganshuang Granules in the treatment of cirrhosis and depression.The Ingenuity Pathway Analysis(IPA)software was conducted to find common pathways and functions of cirrhosis and depression.The relevant targets of Ganshuang Granules for the treatment of two diseases were imported into BioGPS and Genecards database to obtain the tissue distribution and subcellular distribution,and pathway and function annotation analysis were conducted to map the disease treatment network.[Results]The common underlying pathogenesis of cirrhosis and depression may be related to immune dysfunction(dendritic cell maturation,Th1 and Th2 activation pathway,leucocyte migration),inflammation(acute phase response signaling,glucocorticoid receptor signaling,HMGB1 signaling,organ inflammation)and abnormal glucose and lipid metabolism(hepatic cholestasis,glucose metabolic disorder,diabetes).Ganshuang Granules may treat two diseases through immune regulation,anti-inflammation and glucose and lipid metabolism regulation.[Conclusion]The common pathogenesis of cirrhosis and depression involves immune dysfunction,inflammatory response and abnormal glucose and lipid metabolism.Ganshuang Granules can be used to treat two diseases through immune function and glucose and lipid metabolism regulation,which can provide directions for further explanation of the therapeutic effect and pharmacological mechanism.